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›› 2008, Vol. 8 ›› Issue (6): 1179-1184.

• 生化工程专栏 • 上一篇    下一篇

Preliminary Study on E. coli Microbial Fuel Cell and On-electrode Taming of the Biocatalyst

郗名悦 孙彦平   

  1. 太原理工大学 洁净化工研究所 山西太原理工大学洁净化工研究所
  • 收稿日期:2008-07-14 修回日期:2008-10-23 出版日期:2008-12-20 发布日期:2008-12-20
  • 通讯作者: 孙彦平

Preliminary Study on E. coli Microbial Fuel Cell and On-electrode Taming of the Biocatalyst

XI Ming-yue SUN Yan-ping   

  1. Department of Chemical Engineering, Taiyuan University of Technology Taiyuan University of Technology，Taiyuan，Shanxi
  • Received:2008-07-14 Revised:2008-10-23 Online:2008-12-20 Published:2008-12-20
  • Contact: SUN Yan-ping

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摘要： A mediator microbial fuel cell (MFC) was constructed by using E. coli as biocatalyst and new methylene blue as electron mediator. E. coli cells were carried out in anaerobic growth prior to inoculating them into the MFC in order to pre-adapt bacterial metabolism in an anaerobic environment, the electricity generation of MFC was tested, its maximum power density reached 263.94 mW/m2 with the corresponding current density 1287.50 mA/m2, the internal resistance of MFC was 200 W, and capability of the MFC was even better than those reported so far. Moreover, on-electrode taming method was adopted to improve electrochemical activity of E. coli, namely a combination of E. coli taming and electricity generation simultaneously in the same MFC without scraping off the biofilm of MFC, after the 4th on-electrode taming, the tamed E. coli MFC showed a 54% improvement in peak current density, being 612.50 mA/m2, and a 64% improvement in the maximum power output, being 166.67 mW/m2, compared with that of parental E. coli MFC. And the maturation time of tamed biofilm was obviously reduced to 240 min, quickening up 1 times compared with that of parental E. coli biofilm.

关键词: microbial fuel cell, E. coli, taming, biofilm

Abstract: A mediator microbial fuel cell (MFC) was constructed by using E. coli as biocatalyst and new methylene blue as electron mediator. E. coli cells were carried out in anaerobic growth prior to inoculating them into the MFC in order to pre-adapt bacterial metabolism in an anaerobic environment, the electricity generation of MFC was tested, its maximum power density reached 263.94 mW/m2 with the corresponding current density 1287.50 mA/m2, the internal resistance of MFC was 200 W, and capability of the MFC was even better than those reported so far. Moreover, on-electrode taming method was adopted to improve electrochemical activity of E. coli, namely a combination of E. coli taming and electricity generation simultaneously in the same MFC without scraping off the biofilm of MFC, after the 4th on-electrode taming, the tamed E. coli MFC showed a 54% improvement in peak current density, being 612.50 mA/m2, and a 64% improvement in the maximum power output, being 166.67 mW/m2, compared with that of parental E. coli MFC. And the maturation time of tamed biofilm was obviously reduced to 240 min, quickening up 1 times compared with that of parental E. coli biofilm.

Key words: microbial fuel cell, E. coli, taming, biofilm