欢迎访问过程工程学报, 今天是

›› 2008, Vol. 8 ›› Issue (2): 339-344.

• 生化工程专栏 • 上一篇    下一篇

聚维酮碘在硅胶表面的固载化及其杀菌性能

刘青 高保娇 杨莹   

  1. 中北大学化工系 中北大学化工系 中北大学化工系
  • 收稿日期:2007-11-09 修回日期:2008-01-03 出版日期:2008-04-20 发布日期:2008-04-20
  • 通讯作者: 高保娇

Immobilization of Povidone-Iodine on Silica Gel Particles and Sterilization Activity

LIU Qing GAO Bao-jiao YANG Ying   

  1. Department of Chemical Engineering, North University Department of Chemical Engineering, North University Department of Chemical Engineering, North University
  • Received:2007-11-09 Revised:2008-01-03 Online:2008-04-20 Published:2008-04-20
  • Contact: GAO Bao-jiao

摘要: 将N-乙烯基吡咯烷酮(NVP)接枝聚合到硅胶表面,制得接枝微粒PVP/SiO2;使接枝微粒PVP/SiO2在乙醇溶液中与碘发生络合反应,形成水不溶的聚维酮碘PVP-I2/SiO2,即实现了聚维酮碘的固载化. 考察了各种因素对络合反应的影响规律,用红外光谱与化学分析法对功能微粒PVP-I2/SiO2的化学结构与组成进行了表征. 以大肠杆菌为致病菌体,采用平板活菌计数法研究了PVP-I2/SiO2的杀菌性能. 研究结果表明,PVP/SiO2与碘的络合反应的适宜温度为60℃,经12 h后达到络合平衡,络合度随溶液中碘浓度的增大而提高,最大约为0.16%(w). 固载化的功能微粒PVP-I2/SiO2具有很强的杀菌能力,在药剂量为5 g/L的条件下,与浓度为109 CFU/mL的菌悬液接触3 min,即可使杀菌率达100%;胞外DNA和RNA测定与TTC-脱氢酶活性测定结果验证了水不溶聚维酮碘PVP-I2/SiO2的杀菌机理.

关键词: 聚维酮碘, 接枝聚合, 络合反应, 水不溶杀菌材料, 杀菌机理

Abstract: N-vinyl pyrrolidone (NVP) was grafted on silica gel particles, resulting in the grafted particles of poly(pyrrolidone)/SiO2 (PVP/SiO2). The complexation reaction between PVP/SiO2 and iodine was conducted in ethanol as solvent. The immobilization of povidone-iodine on SiO2 was realized, and the functional particles of PVP-I2/SiO2 were obtained. The effects of various influential factors on the complexation reaction were examined. The structure and composition of the functionalized particles of PVP-I2/SiO2 were characterized with Fourier transform infrared spectrum and chemical analysis method. The sterilization property of PVP-I2/SiO2 was studied with Escherichia coil as a disease-leading bacterium and the method of live bacteria plate counting. The experimental results show that the suitable temperature for the complexation reaction is 60℃, and the complexation degree increases with the increase of iodine concentration. At 60℃ a maximum complexation degree of 0.16%(w) is obtained as iodine is dissolved in ethanol in saturated state. The PVP-I2/SiO2 particles have strong sterilization activity. Their sterilization rate can reach 100% as they contact with the cell suspension, which has a concentration of 1′109 CFU/mL, only at a dosage of 5 g/L and for 3 min. TTC-dehydrogenase determination and measurement of extracellular DNA and RNA have fully verified the sterilization mechanism of PVP-I2/SiO2.

Key words: povidone-iodine, grafting polymerization, complexation reaction, water insoluble antibacterial material, sterilization mechanism

中图分类号: