欢迎访问过程工程学报, 今天是

›› 2002, Vol. 2 ›› Issue (6): 0-0.

• 6 •    

酶法转化谷氨酰胺

杨春玉,马翠卿,许平,张兆斌,李金山   

  1. 山东大学微生物技术国家重点实验室,山东 济南 250100
  • 出版日期:2002-12-20 发布日期:2002-12-20

Glutamine Production by the Enzyme Method

YANG Chun-yu, MA Cui-qing, XU Ping, ZHANG Zhao-bin, LI Jin-shan   

  1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong 250100, China
  • Online:2002-12-20 Published:2002-12-20

摘要: 高产谷氨酰胺的谷氨酸棒杆菌经酶解和超声波处理后,释放出高活性的谷氨酰胺合成酶(Glutamine synthetase,简称GS). GS的胞外催化反应受各种反应条件和因素的影响. 通过单因子实验、响应面实验和正交实验发现,反应混合物中最显著的影响是腺三膦(ATP),当ATP浓度在40~50 mmol/L时,催化作用达到最高;同时酶量对反应也有较大的影响. 研究了粗酶液在细胞外反应时各种因素对谷氨酸棒杆菌GS催化作用的影响,获得了体外酶催化反应的适宜条件,最高转化产生的谷氨酰胺可达7.1 g/L,转化率达到94.8%.

关键词: 谷氨酰胺合成酶, ATP, 正交实验

Abstract: After enzymatic lysis and ultrasonication, Corynebacterium glutamicum, which produces high yield of glutamine, can release glutamine synthetase (GS) with high activity. The GS extracellular catalysis is regulated by a few conditions and factors. By mono-factor experiment, response experiment and orthogonal experiment, the suitable conditions for GS extracellular catalysis are determined: in the reaction mixture, the most significant factor is ATP, and the concentration of ATP is 40~50 mmol/L. The amount of enzyme also shows strong effect to the reaction. Using the cell-free extracts, the effects of some factors on the GS conversion in the extracellular reaction are examined and the optimal condition for GS extracellular catalysis obtained. The highest conversion is 7.1 g/L and the conversion yield is 94.8%.

Key words: GS, ATP, orthogonal experiment

中图分类号: