塑料薄膜材料在微藻培养环境中的稳定性和生物附着行为

doi:10.12034/j.issn.1009-606X.219175

过程工程学报 ›› 2020, Vol. 20 ›› Issue (1): 74-83.DOI: 10.12034/j.issn.1009-606X.219175

塑料薄膜材料在微藻培养环境中的稳定性和生物附着行为

王艺璇^1,2，颜成虎^1*，丛威^1*

1. 中国科学院过程工程研究所生化工程国家重点实验室，北京 100190 2. 中国科学院大学化学工程学院，北京 100049

收稿日期:2019-04-08 修回日期:2019-04-30 出版日期:2020-01-22 发布日期:2020-01-14
通讯作者: 王艺璇 iwangyx@163.com
基金资助:
抗污光生物反应器表面的低成本构建方法及作用机制;沙化土地稳定恢复的生物质新材料筛选及产业化技术

Stability and biofouling behavior of plastic films in microalgae cultivation

Yixuan WANG^1,2, Chenghu YAN^1*, Wei CONG^1*

1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 2. School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

Received:2019-04-08 Revised:2019-04-30 Online:2020-01-22 Published:2020-01-14

摘要/Abstract

摘要： 考察了6种薄膜材料在次氯酸钠(NaClO)溶液、HCl溶液(pH=2)和NaOH溶液(pH=12)中的稳定性及其在普通小球藻培养体系中的生物附着行为。结果表明，聚氯乙烯(PVC)在3种溶液中浸泡后420 nm处的透光率下降约50%，聚氨酯(PU)在NaClO溶液中、乙烯?醋酸乙烯酯(EVA)在NaOH溶液中浸泡后420 nm处的透光率下降10%?15%，聚乙烯(PE)、聚丙烯(PP)和聚对苯二甲酸乙二醇酯(PET)在3种溶液中浸泡后的透光率没有明显变化；6种薄膜材料在普通小球藻培养体系中均有微藻明显附着，浸泡7 d后透光率明显下降，其中PVC表面附着最严重，浸泡7 d后透光率接近0，附着物量随浸泡时间延长而增加，浸泡45 d后达3069 ?g/cm2；其它5种薄膜表面附着物量先增加后降低，PU和EVA在第15 d时、PE, PP和PET在第30 d时附着物量最大，分别为292, 375, 292, 194和236 ?g/cm2。

关键词: 光生物反应器, 薄膜材料, 小球藻, 稳定性, 微藻附着

Abstract: The stability of six typical kinds of plastic films including polyethylene (PE), polypropylene (PP), ethylene/vinyl acetate (EVA), polyvinyl chloride (PVC), polyurethane (PU) and polyethylene terephthalate (PET) in NaClO, NaOH and HCl solutions and biofouling behavior in microalgae cultivation (Chlorella vulgaris) system were studied. The results showed that PVC film performed the poorest stability, and the transmittance decreased about 50% after immersing in NaClO, NaOH and HCl solutions. The transmittance of PU film and EVA film declined 10%?15% in NaClO and NaOH solutions, respectively. The transmittance of the other three kinds of plastic films has no significant change when immersed in NaClO, NaOH and HCl solutions for 24 h. Obvious microalgae biofouling behavior happened in six kinds of films, which was a typical biofilm formation process. Adhesion behavior of PVC surface was the most distinct, and the transmittance declined to about 0 on the 7th day, and the amount of adsorption solids increased with time and reached 3069 ?g/cm2 on the 45th day. However, the amount of adsorption solids on PU, EVA, PE, PP and PET film surface increased sharply and then decreased with time, the maximum amounts were 292, 375, 292, 194 and 236 ?g/cm2, respectively. The increase of chlorophyll content on the surface of the plastic films was not obvious in the first 4 d, which indicated that the initial stage of the adhesion was mainly the adhesion of protein and extracellular polymeric substances (EPS), and then microalgae cells began to adhere. The attachment of protein, EPS and microalgae cells on the plastic films was a typical biofilm formation process. The adhesion behavior of microalgae was affected by chemical structure and surface properties of the films, such as hydrophilicity, roughness and surface charge. For Chlorella vulgaris, polyolefin (PE, PP, etc.) and PET film surface with high hydrophilicity, low roughness and negatively charged will perform good anti-biofouling properties.

Key words: Photobioreactor, Plastic films, Chlorella sp., Stability, Biofouling

王艺璇颜成虎丛威. 塑料薄膜材料在微藻培养环境中的稳定性和生物附着行为[J]. 过程工程学报, 2020, 20(1): 74-83.

Yixuan WANG Chenghu YAN Wei CONG. Stability and biofouling behavior of plastic films in microalgae cultivation[J]. Chin. J. Process Eng., 2020, 20(1): 74-83.

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塑料薄膜材料在微藻培养环境中的稳定性和生物附着行为

Stability and biofouling behavior of plastic films in microalgae cultivation

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