Effect of Rare Earth Elements on Fermentation Process of 2-Keto-L-gulonic Acid
ZHENG Qizo-shuang， ZHOU Ling-wen CHEN Fu-jiang， LIU Jie LI Zhen CHEN Jian,
State Key Lab of Food Science and Technology, Jiangnan University State Key Lab of Food Science and Technology, Jiangnan University State Key Lab of Food Science and Technology, Jiangnan University Jiangsu Jiangshan Pharmaceutical Co., Ltd. Lab of Biotechnology, School of Biotechnology, Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
Abstract：The effects of different rare earth ions and coordination compounds on the synthesis of 2-keto-L-gulonic acid (2-KLG) by mixed culture of Bacillus megaterium and Ketogulonigenium vulgare were investigated in the two-step fermentation of vitamin C. The results indicated that the proper promotion concentrations of various rare earth ions were all about 5 mmol/L. Furthermore, the low concentration caused 2-KLG synthesis promotion while high concentration caused 2-KLG synthesis inhibition. EDTA-La and lanthanum citrate inhibited 2-KLG synthesis, but lanthanum oxalate promoted it at a concentration of 1 mmol/L and enhanced the final production of 2-KLG by 6.4%. The cell growth conditions at the mid- and late fermentation stages were remarkably improved with the addition of 5 mmol/L La3+. 2-KLG ultimate output was increased and the fermentation time reduced at the same time. When 5 mmol/L La3+ was fed to 3.6 L fermentor for batch fermentation, the L-sorbose consumption rate and 2-KLG productivity were increased by 12.4% and 14.8% compared with that of the control, respectively. And the fermentation time was declined by 11.1%.
郑巧双 周景文 陈孚江 刘杰 刘立明 陈坚. 稀土元素对2-酮基-L-古龙酸发酵过程的影响[J]. , 2010, 10(4): 750-755.
ZHENG Qizo-shuang; ZHOU Ling-wen CHEN Fu-jiang; LIU Jie LI Zhen CHEN Jian;. Effect of Rare Earth Elements on Fermentation Process of 2-Keto-L-gulonic Acid. , 2010, 10(4): 750-755.