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›› 2010, Vol. 10 ›› Issue (4): 750-755.

• 生化工程专栏 • 上一篇    下一篇

稀土元素对2-酮基-L-古龙酸发酵过程的影响

郑巧双 周景文 陈孚江 刘杰 刘立明 陈坚   

  1. 江南大学食品科学与技术国家重点实验室 江南大学食品科学与技术国家重点实验室 江南大学食品科学与技术国家重点实验室 江苏江山制药有限公司 江南大学生物工程学院环境生物技术实验室 江南大学工业生物技术教育部重点实验室
  • 收稿日期:2010-05-07 修回日期:2010-08-04 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 刘立明

Effect of Rare Earth Elements on Fermentation Process of 2-Keto-L-gulonic Acid

ZHENG Qizo-shuang, ZHOU Ling-wen CHEN Fu-jiang, LIU Jie LI Zhen CHEN Jian,   

  1. State Key Lab of Food Science and Technology, Jiangnan University State Key Lab of Food Science and Technology, Jiangnan University State Key Lab of Food Science and Technology, Jiangnan University Jiangsu Jiangshan Pharmaceutical Co., Ltd. Lab of Biotechnology, School of Biotechnology, Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
  • Received:2010-05-07 Revised:2010-08-04 Online:2010-08-20 Published:2010-08-20
  • Contact: LI Zhen

摘要: 采用普通生酮基古龙酸菌(Ketogulonigenium vulgare)与巨大芽孢杆菌(Bacillus megaterium)混合菌系,研究了不同稀土离子及其配合物对发酵生产维生素C前体物质2-酮基-L-古龙酸(2-KLG)的影响. 结果表明,各稀土离子对2-KLG合成的最佳促进浓度均约为5 mmol/L,且具有低浓度促进、高浓度抑制的作用规律. EDTA镧、柠檬酸镧均抑制2-KLG的合成,而1 mmol/L草酸镧对其合成代谢有促进作用,使其产量提高6.4%. 摇瓶中添加5 mmol/L La3+,发酵中后期菌体生长状况得到显著改善的同时提高了2-KLG最终产量并缩短了发酵周期. 3.6 L发酵罐中添加5 mmol/L La3+进行分批发酵,平均山梨糖消耗速率和2-KLG生产强度比对照分别提高12.4%和14.8%,发酵周期缩短11.1

关键词: 二步发酵, 稀土元素, 2-酮基-L-古龙酸, 稀土配合物

Abstract: The effects of different rare earth ions and coordination compounds on the synthesis of 2-keto-L-gulonic acid (2-KLG) by mixed culture of Bacillus megaterium and Ketogulonigenium vulgare were investigated in the two-step fermentation of vitamin C. The results indicated that the proper promotion concentrations of various rare earth ions were all about 5 mmol/L. Furthermore, the low concentration caused 2-KLG synthesis promotion while high concentration caused 2-KLG synthesis inhibition. EDTA-La and lanthanum citrate inhibited 2-KLG synthesis, but lanthanum oxalate promoted it at a concentration of 1 mmol/L and enhanced the final production of 2-KLG by 6.4%. The cell growth conditions at the mid- and late fermentation stages were remarkably improved with the addition of 5 mmol/L La3+. 2-KLG ultimate output was increased and the fermentation time reduced at the same time. When 5 mmol/L La3+ was fed to 3.6 L fermentor for batch fermentation, the L-sorbose consumption rate and 2-KLG productivity were increased by 12.4% and 14.8% compared with that of the control, respectively. And the fermentation time was declined by 11.1%.

Key words: two-step fermentation, rare earth ions, 2-keto-L-gulonic acid, rare earth complex

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