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›› 2005, Vol. 5 ›› Issue (3): 327-331.

• 生化工程专栏 • 上一篇    下一篇

产朊假丝酵母流加发酵法生产谷胱甘肽

卫功元,李寅,堵国成,陈坚   

  1. 苏州大学生命科学学院生物工程教研室生物工程教研室
  • 出版日期:2005-06-20 发布日期:2005-06-20

Fed-batch Fermentative Production of Glutathione by Candida utilis

WEI Gong-yuan,LI Yin,DU Guo-cheng,CHEN Jian   

  1. School of Life Science, Soochow University
  • Online:2005-06-20 Published:2005-06-20

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摘要： 研究了产朊假丝酵母在不同葡萄糖浓度下的谷胱甘肽(GSH)分批发酵过程，在此基础上进一步考察了重复补料、恒速流加和指数流加等不同培养方式对GSH发酵生产的影响. 结果发现，这几种培养方式都可以实现酵母细胞和GSH的高产. 综合比较，无论是从细胞还是从GSH的产量、得率和生产强度的角度来看，指数流加都是较为理想的选择. 经过48 h的指数流加培养，细胞干重达到40.9 g/L， GSH产量和胞内GSH含量分别达到857.2 mg/L和2.25%.

关键词: 谷胱甘肽, 产朊假丝酵母, 流加发酵, 生产

Abstract: The batch cultivation processes of C. utilis WSH 02-08 in a 7 L stirred fermentor for GSH production under diverse initial glucose concentrations were investigated. It was concluded that high concentration, high yield and high productivity of both yeast cell and GSH could not be consolidated in a batch process. Based on the results, the effects of different cultivation modes such as batch feeding, constant-rate feeding and exponential rate feeding on GSH production were also studied. High production of yeast cell and GSH were all realized in these cultivation modes of glucose feeding. As a whole, fed-batch culture with exponential rate feeding was the most suitable approach to attain high concentrations, high yields and high productivities of yeast cells and GSH in the cultivation of C. utilis WSH 02-08, under which the maximum DCW, GSH and intracellular GSH content reached 40.9 g/L, 857.2 mg/L and 2.25%, respectively, at the culture time of 48 h.

Key words: glutathione, Candida utilis, fed-batch fermentation, production