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›› 2003, Vol. 3 ›› Issue (4): 0-0.

• 4 •    

产烃葡萄藻在气升式光生物反应器中的分批补料培养

王军,杨素玲,丛威,蔡昭铃   

  1. 中国科学院过程工程研究所生化工程国家重点实验室,北京 100080
  • 出版日期:2003-08-20 发布日期:2003-08-20

Fed-batch Cultivation of Botryococcus braunii in an Air-lift Photobioreactor

WANG Jun, YANG Su-ling, CONG Wei, CAI Zhao-ling   

  1. 1. State Key Lab. Biochem. Eng., Inst. Chem. Metall., Chinese Academy of Sciences, Beijing 100080, China; 2. College of Life Science, Hubei University, Wuhan, Hubei 430062, China
  • Online:2003-08-20 Published:2003-08-20

摘要: 在3 L气升式光生物反应器中进行了产烃葡萄藻的培养. 批式培养时,葡萄藻消耗氮、磷的速度较快,培养液中氮、磷营养盐的缺乏限制了藻细胞进一步生长和增殖. 采取分批补料方式,使培养液中KNO3和K2HPO4浓度分别维持在100和30 mg/L,可克服氮、磷限制问题. 与批式培养相比,生物量从1.3 g/L增加到1.9 g/L,烃含量从占细胞干重的22%提高到29%,从而使烃产量从0.286 g/L增加到0.551 g/L,提高了92.6%.

关键词: 葡萄藻(Botryococcus braunii), 气升式光生物反应器, N, P, 分批补料

Abstract: Bacteriocin nisin was isolated and purified in temperature-induced aqueous two-phase systems using the capacity of extracting hydrophobic proteins of TritonX-114. The concentrations of sodium chloride and TritonX-114 which can form the aqueous two-phase systems were optimized by the response surface method. The full-factorial design was used to assess the main effects of factors and determine the path of steepest ascent to the optimal region of the aqueous two-phase system composition. The optimum concentrations of TritonX-114 and sodium chloride were then easily determined by using a central composite design and were found to be 3.27% of TritonX-114 and 0.85 mol/L sodium chloride. The recovery and purification factor were 92% and 9.05 respectively under the optimum conditions.

Key words: Botryococcus braunii, air-lift photobioreactor, nitrate, phosphate, fed-batch

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