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›› 2002, Vol. 2 ›› Issue (3): 0-0.

• 3 •    

黄孢原毛平革菌合成的木质素过氧化物酶、锰过氧化物酶及其菌球在染料脱色过程中的作用

章燕芳,李华钟,华兆哲,陈坚   

  1. 江南大学生物工程学院工业生物技术教育部重点实验室，江苏 无锡 214036
  • 出版日期:2002-06-20 发布日期:2002-06-20

Roles of Lignin Peroxidase, Manganese Peroxidase and Pellet of Phanerochaete chrysosporium in Decoloration of Dyes

ZHANG Yan-fang, LI Hua-zhong, HUA Zhao-zhe, CHEN Jian   

  1. Key Laboratory Industrial Biotechnology, Ministry of Education, Biotechnology School, Southern Yangtze University, Wuxi, Jiangsu 214036, China
  • Online:2002-06-20 Published:2002-06-20

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摘要： 在黄孢原毛平革菌(Phanerochaete chrysosporium)限氮浸没式培养中，获得了含木质素过氧化物酶(LiP)和锰过氧化物酶(MnP)、只含LiP或MnP以及无LiP和MnP的4种培养物，考察了LiP，MnP及菌球在染料脱色过程中的作用. 结果表明，4种培养物对6种染料都有明显的脱色效果，甲基橙、橙I、次甲基蓝脱色率在90%左右，刚果红、直接湖蓝脱色率在80%左右，结晶紫脱色率在60%左右. 无酶情况下染料的脱色主要是菌球的吸附，有酶情况下染料的脱色主要是酶的降解. 菌球在脱色过程中除了吸附染料外，还起到提供H2O2和藜芦醇的作用，促进了染料的降解.

关键词: 黄孢原毛平革菌, 木质素过氧化物酶, 锰过氧化物酶, 菌球, 染料, 脱色

Abstract: 4 cultures containing LiP and MnP, LiP only, MnP only , neither LiP nor MnP were obtained respectively in N-limited submerged culture of Phanerochaete chrysosporium and the roles of LiP, MnP and pellet in decoloration of dyes were investigated. All 4 cultures have considerable decoloration effect on 6 dyes: about 90% decoloration rate for methyl orange, orange I, methylene blue, 80% for congo red, direct blue and 60% for crystal violet. Biodegradation and absorption play main role in the process of decoloration with and without enzymes respectively. The pellet provided H2O2 and veratryl alcohol besides the absorption of dyes, which promoted the degradation of dyes.

Key words: Phanerochaete chrysosporium, lignin peroxidase, manganese peroxidase, pellet, dyes, decoloration

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