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›› 2009, Vol. 9 ›› Issue (6): 1153-1158.

• 生化工程专栏 • 上一篇    下一篇

离子色谱-直接电导法同时测定常见氨基酸和无机阳离子

焦扬 杨鹏波 李春 丛威   

  1. 石河子大学食品学院 中国科学院过程工程研究所生化工程国家重点实验室 北京理工大学生命科学与技术学院生物技术系 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2009-06-04 修回日期:2009-08-05 出版日期:2009-12-20 发布日期:2009-12-20
  • 通讯作者: 杨鹏波

Simultaneous Determination of Amino Acids and Inorganic Cations by Ion Chromatography with Direct Conductivity Detection

JIAO Yang YANG Peng-bo LI Chun CONG Wei   

  1. Food College, Shihezi University State Key Lab of Biochemical Engineering，Institute of Process Engineering，Chinese Academy of Sciences Department of Biotechnology, School of Life Science and Technology, Beijing Institute of Technology State Key Lab. Biochem. Eng., Inst. Process Eng., CAS
  • Received:2009-06-04 Revised:2009-08-05 Online:2009-12-20 Published:2009-12-20
  • Contact: YANG Peng-bo

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摘要： 采用阳离子交换色谱-直接电导法测定部分常见氨基酸和无机阳离子，以酒石酸和吡啶二羧酸溶液为淋洗液，利用阳离子色谱系统等度洗脱，通过对色谱分析条件的优化，无需衍生化即可同时测定谷氨酸、丙氨酸、甲硫氨酸、亮氨酸、苯丙氨酸、赖氨酸及Na+, NH4+, K+, Ca2+和Mg2+. 选择12 min内出峰的5种氨基酸和3种无机阳离子进行定量分析，结果表明，其相对标准偏差RSDn=5≤4.690%，标准曲线的线性相关系数不低于0.9984，检出限在0.27~10.34 mg/L之间. 以建立的方法测定味精等电母液中的氨基酸，结果显示，氨基酸的回收率为88.7%~107.2%.

关键词: 离子色谱, 直接电导检测器, 氨基酸, 无机阳离子

Abstract: A simple, rapid and accurate method for simultaneous determination of six amino acids (glutamic acid, alanine, methionine, leucine, phenylalanine and lysine) and five inorganic cations (Na+, NH4+, K+, Ca2+ and Mg2+) was developed. The quantitative determination of these ions was accomplished by ion chromatography with direct conductivity detection based on their ionization in acidic medium without chemical suppression and derivatization. In a single chromatographic run, the five amino acids (glutamic acid, alanine, methionine, leucine and phenylalanine) and three inorganic cations (Na+, NH4+ and K+) were separated and determined within 12 min. The relative standard deviation (RSD, n=5) was less than 4.690%. The detection limits calculated were in the range from 0.27 to 10.34 mg/L. All the correlation coefficients (R2) of these ions were not less than 0.9984. The recovery rates of some amino acids in isoelectric supernant of monosodium glutamate were in the range from 88.7% to 107.2%.

Key words: ion chromatography, direct conductivity detection, amino acid, inorganic cation

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