欢迎访问过程工程学报, 今天是

›› 2010, Vol. 10 ›› Issue (5): 976-980.

• 生化工程专栏 • 上一篇    下一篇

Actinobacillus succinogenes NJ113产丁二酸过程中的底物抑制

方晓江 李建 郑晓宇 奚永兰 张敏 张煜 陈可泉 韦萍 姜岷   

  1. 南京工业大学生物与制药工程学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院 南京工业大学制药与生命科学学院
  • 收稿日期:2010-07-08 修回日期:2010-09-20 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 姜岷

Substrate Inhibition of Succinic Acid Fermentation with Actinobacillus succinogenes NJ113

FANG Xiao-jiang, LI Jian, ZHENG Xiao-yu, XI Yong-lan, ZHANG Min， ZHANG Yi， CHEN Ke-quan WEI Ping ＣＨＥＮＧ　   

  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of TechnologyCollege of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology 南京工业大学食品与轻工学院 College of Life Science and Pharmacy, Nanjing University of Technology
  • Received:2010-07-08 Revised:2010-09-20 Online:2010-10-20 Published:2010-10-20
  • Contact: ＣＨＥＮＧ　

PDF

摘要： 研究了分批发酵条件下以葡萄糖作为底物对产琥珀酸放线杆菌Actinobacillus succinogene NJ113发酵产丁二酸的影响，针对底物抑制现象，采用变速补料控制发酵罐中葡萄糖浓度的补料分批发酵方式. 结果表明，发酵过程中将葡萄糖浓度控制在0~10 g/L，以Na2CO3作为pH调节剂，经26 h厌氧发酵，消耗60 g/L葡萄糖，能积累45.27 g/L丁二酸，得率达75.45%，生产强度为1.74 g/(L×h)，比初始葡萄糖浓度为60 g/L的分批发酵周期缩短了18.75%，主产物丁二酸的得率和生产强度分别提高了5.44%和31.82%，副产物甲酸产量有所减少，而乙酸产量有所增加. 通过代谢网络中相关酶的酶活分析，解析了补料过程中主副产物的分布.

关键词: 丁二酸, 底物抑制, 补料分批发酵, Actinobacillus succinogenes

Abstract: Anaerobic fermentative production of succinic acid by Actinobacillus succinogenes NJ113 with glucose as the substrate in batch fermentation was studied. Fed-batch fermentation was performed to minimize the inhibition effect of substrate (glucose). In this case, 45.27 g/L succinic acid and productivity of 1.74 g/(L×h) were attained after 26 h incubation with total glucose concentration of 60 g/L using Na2CO3 as the pH regulator, with a level less than 10 g/L of glucose concentration. Compared with the batch fermentation, the fermentation period was already shortened by up to 18.75%. The yield of succinic acid and productivity were raised by 5.44% and 31.82% respectively in fed-batch fermentation, and the by-product formic acid decreased and the acetic acid increased in the process.

Key words: succinic acid, substrate inhibition, fed-batch fermentation, Actinobacillus succinogenes

中图分类号: