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›› 2005, Vol. 5 ›› Issue (4): 425-429.

• 生化工程专栏 • 上一篇    下一篇

1,3-丙二醇分批发酵动力学模型

程可可,林日辉,刘宏娟,刘德华   

  1. 清华大学化学工程系
  • 出版日期:2005-08-20 发布日期:2005-08-20

Kinetic Analysis of Anaerobic Fermentation of 1,3-Propanediol by Klebsiella pneumoniae

CHEN Ke-ke,LIN Ri-hui,LIU Hong-juan,LIU De-hua   

  1. Department of Chemical Engineering, Tsinghua Universit
  • Online:2005-08-20 Published:2005-08-20

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被引次数

54

摘要： 在分批发酵中，研究了Klebsiella pneumoniae的生长、底物甘油消耗及1,3-丙二醇的产生特性. 基于Logistic方程和Luedeking-Piret方程，得到了描述1,3-丙二醇分批发酵过程的动力学模型及模型参数，该组模型能很好地拟合发酵过程，并在初始甘油浓度变化较大的范围内表现出很好的适用性. 同时，所建立的模型也基本反映了Klebsiella pneumoniae分批发酵过程的动力学特征. 基于分批发酵动力学模型，提出了以甘油为单一碳源时的底物流加策略，通过与其他流加策略条件下的发酵对比实验表明，通过基于动力学模型的流加策略可获得更高的1,3-丙二醇浓度及生产强度.

关键词: 动力学模型, 3-丙二醇, Klebsiella pneumoniae, 发酵

Abstract: The fermentation process of 1,3-propanediol by Klebsiella pneumoniae with glycerol as substrate was studied in batch system. A kinetic model was proposed based on the Logistic and Luedeking-Piret equations of cell growth, product formation and substrate consumption. With the evaluated model parameters, the model could provide reasonable description for the fermentation process under various conditions. Based on the model, a feeding strategy for glycerol was determined to maximize the final 1,3-propanediol concentration. The experimental results showed that the feeding mode with nonlinear optimization could improve the 1,3-propanediol productivity and concentration compared with other feeding strategies, such as pulse feeding and constant glycerol concentration feeding.

Key words: kinetics, 3-propanediol, Klebsiella pneumoniae, fermentation