蔗糖、乳酸及KH2PO4对乳链菌肽合成的影响

›› 2004, Vol. 4 ›› Issue (1): 28-31.

蔗糖、乳酸及KH2PO4对乳链菌肽合成的影响

吕文华,丛威,蔡昭铃

中国科学院过程工程研究所生化工程国家重点实验室

出版日期:2004-02-20 发布日期:2004-02-20

Effects of Sucrose, Lactate and KH2PO4 on Nisin Production by L. lactis sp. lactis ATCC11454

LU Wen-hua,CONG Wei,CAI Zhao-ling

中国科学院过程工程研究所生化工程国家重点实验室

Online:2004-02-20 Published:2004-02-20

摘要/Abstract

摘要： 在恒pH、厌氧条件下，研究了蔗糖、乳酸及KH2PO4对L. lactis sp. lactis ATCC11454分批发酵中细胞生长和乳链菌肽合成的影响. 乳链菌肽的合成表现为初级代谢动力学特征. 蔗糖对乳链菌肽的合成具有调控作用，提高蔗糖浓度可以提高细胞浓度及乳链菌肽效价，但过高的蔗糖浓度(>30 g/L)抑制了乳链菌肽的合成. 乳酸作为代谢产物抑制了菌体的生长，同时导致乳链菌肽效价的下降，乳链菌肽对菌体的得率系数YN/X随起始乳酸浓度的增加而减小，表明乳酸对乳链菌肽合成的影响更大. KH2PO4在恒pH条件下对乳链菌肽的合成并没有促进作用.

关键词: 乳链菌肽, 蔗糖, 乳酸, KH2PO4

Abstract: The effects of sucrose, lactate and KH2PO4 on cell growth and nisin production kinetics of L. lactis sp. lactis ATCC11454 were investigated in pH-controlled batch culture under anaerobic conditions. Nisin production showed primary metabolite kinetics. Sucrose had the effect of regulation on nisin production, higher concentration of sucrose yielded higher biomass and nisin titer. However, the nisin production decreased when sucrose concentration was greater than 30 g/L. Lactate as the metabolic product inhibited both the cell growth and nisin production. YN/X, the nisin production yield based on sucrose consumed decreased with the increase of initial lactate concentration, which indicated that the effect of lactate on nisin production was higher than the effect on cell growth. The improvement of nisin production by adding KH2PO4 was not observed in batch culture at controlled pH 6.80.

Key words: nisin, sucrose, lactate, KH2PO4

中图分类号:

工艺

吕文华;丛威;蔡昭铃. 蔗糖、乳酸及KH2PO4对乳链菌肽合成的影响[J]. , 2004, 4(1): 28-31.

LU Wen-hua;CONG Wei;CAI Zhao-ling. Effects of Sucrose, Lactate and KH2PO4 on Nisin Production by L. lactis sp. lactis ATCC11454[J]. , 2004, 4(1): 28-31.

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