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›› 2005, Vol. 5 ›› Issue (5): 540-544.

• 生化工程专栏 • 上一篇    下一篇

分段通气对Klebsiella pneumoniae生产1,3-丙二醇关键酶和辅酶的影响

杜晨宇,刘铭,饶治,包晓玮,沈金玉,曹竹安   

  1. 清华大学化学工程系生物化工研究所
  • 出版日期:2005-10-20 发布日期:2005-10-20

Effect of Alternative Aeration on Key Enzymes and Coenzymes in 1,3-Propanediol Production by Klebsiella pneumoniae

DU Chen-yu,LIU Ming,RAO Zhi,BAO Xiao-wei,SHEN Jin-yu,CAO Zhu-an   

  1. Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University
  • Online:2005-10-20 Published:2005-10-20

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摘要： 通过考察1,3-丙二醇合成中关键酶、辅酶的变化，研究了分段通入空气对Klebsiella pneumoniae厌氧发酵生产1,3-丙二醇的影响. 与对照组相比，在发酵前期(12 h)通入空气4 h后，甘油脱氢酶酶活提高1.5倍，1,3-丙二醇氧化还原酶酶活提高18%，1,3-丙二醇浓度提高16%；发酵后期(28和48 h)通入空气后，甘油脱氢酶酶活不变，1,3-丙二醇氧化还原酶酶活下降，1,3-丙二醇浓度降低. 发酵前期，通气对辅酶NADH和NAD的浓度无影响；发酵后期，菌体生长停滞，辅酶的浓度也随之下降.

关键词: 3-丙二醇, Klebsiella pneumoniae, 甘油脱氢酶, 3-丙二醇氧化还原酶, 间歇通气

Abstract: The effect of air in alternative aeration model on 1,3-propandiol anaerobic biosynthesis was studied by examining the activities of key enzymes and coenzymes in Klebsiella pneumoniae. Aerating air in the fore stage (the 12 h) for 4 h, the activities of glycerol dehydrogenase and 1,3-PD oxidoreductase were 1.5 times and 18% higher than control respectively, and the concentration of 1,3-propanediol was 16% higher than control. Aerating air in the later stage (the 28 and the 48 h), the activity of glycerol dehydrogenase changed little, while the activity of 1,3-PD oxidoreductase and 1,3-PD concentration both declined. In the fore stage, the aeration had no effect on the concentrations of NAD and NADH, while in the later stage cell growth ceased, and the concentrations of NAD and NADH declined consequently.

Key words: 3-propanediol, Klebsiella pneumoniae, glycerol dehydrogenase, 3-propanediol oxidoreductase, alternative aeration