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›› 2006, Vol. 6 ›› Issue (5): 809-813.

• 生化工程专栏 • 上一篇    下一篇

高产量、高分子量透明质酸发酵条件优化

成霞,刘登如,陈坚,堵国成   

  1. 江南大学生物工程学院环境生物技术研究室
  • 出版日期:2006-10-20 发布日期:2006-10-20

Optimization of Fermentation Conditions of High Yield and High Molecular Weight Hyaluronic Acid

CHENG Xia,LIU Deng-ru,CHEN Jian,DU Guo-cheng   

  1. Lab of Environmental Biotechnology, School of biotechnology Southern Yangtze University
  • Online:2006-10-20 Published:2006-10-20

摘要: 研究了搅拌转速、初糖浓度及通气量对兽疫链球菌Streptococcus zooepidemicus WSH24发酵生产透明质酸的影响. 研究结果表明,搅拌转速对透明质酸产量及分子量影响很大,搅拌转速为200 r/min时透明质酸产量达到5.3 g/L,平均分子量达到1.88×106 Da,产率系数为0.13 g/g;初始葡萄糖浓度为65.8 g/L时有利于透明质酸的生产,产量达5.9 g/L,平均分子量达1.90×106 Da,产率系数为0.17 g/g;通气量对透明质酸的发酵也有较大影响,通气量为1.2 L/(min·L)时透明质酸的产量及分子量均高于0.5 L/(min·L)时的发酵结果.

关键词: 透明质酸, 兽疫链球菌, 发酵条件优化

Abstract: The effects of initial glucose concentration, agitation speed and aeration rate on hyaluronic acid (HA) production by Streptococcus zooepidemicus WSH24 were investigated in batch culture. The agitation speed had the most pronounced effect on HA yield and molecular weight. Under the condition of 200 r/min, the HA yield, YHA/C and molecular weight could reach 5.3 g/L, 0.13 g/g and 1.88×106 Da, respectively. When the initial glucose concentration was 65.8 g/L, the HA yield, YHA/C and molecular weight reached 5.9 g/L, 0.17 g/g and 1.90×106 Da, respectively. The aeration rate also affected HA fermentation. HA yield and molecular weight were higher at aeration rate of 1.2 L/(min·L) than those at 0.5 L/(min·L).

Key words: hyaluronic acid, Streptococcus zooepidemicus, fermentation condition optimization