Abstract：Desulfurization related genes of a dibenzothiophene desulfurizing bacterium Rhodococcus sp. LY822 were separately amplified via polymerase chain reaction with specific primers based on the related sequences of Rhodococcus erythropolis IGTS8, and dszA, dszB and dszC were cloned. Three expression plasmids, pETA, pETB and pETC, were constructed and transformed into E. coli BL21 strain. After the isopropyl-beta-d-thiogalactopyranoside induction with them, dszA, dszB and dszC were expressed effectively in the recombinant E. coli BL21 strain. The SDS-PAGE results indicated that the molecular weights of desulfurization related genes expression products were about 50, 40 and 45 kDa. Desulfurization activity analysis showed that BL21(DE3)(pETC) cell-free extracts could convert 0.02 mmol/L DBT into DBTO2. BL21(DE3)(pETA) cell-free extracts could convert 0.01 mmol/L DBTO2 into HBPS. BL21(DE3)(pETA) and BL21(DE3)(pETB) cell-free extracts could convert 0.01 mmol/L DBTO2 into 2-HBP. It could be concluded that Rhodococcus sp. LY822 could specially break the C?S bond of dibenzothiophene and convert dibenzothiophene into 2-hydrobenzophene by "4S" biodesulfurization pathway.
白雪晶;熊小超;姜声华;刘会洲;李信. 脱硫菌Rhodococcus sp.LY822专一性脱硫活性及相关基因的研究[J]. , 2008, 8(1): 125-129.
BAI Xue-jing;xiong-xiao-chao;JIANG Sheng-hua;LIU Hui-zhou;LI Xin. Study on Specific Desulfurization Activity and Related Genes of Rhodococcus sp. LY822. , 2008, 8(1): 125-129.
2008, Vol. 8 
