Preparation and Property Regulation of Charge-Collagen Double Acting Microcarrier
WANG Shao-jiu WANG Qi-bao LI Juan MA Guang-hui ZHOU Wei-qing,
College of Chemical and Environmental Engineering,China University of Mining and Technology College of Chemical and Environmental Engineering,China University of Mining and Technology National Key Lab. Biochem. Eng., Institute of Process Engineering, Chinese Academy of Sciences State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences
Abstract：Konjac glucomannan microspheres were firstly aminated with 2-diethylaminoethyl chloride hydrochloride (DEAE), and activated with 1,4-butanediol diglycidyl ether. Then the charge-collagen double acting microcarrier was prepared via the coupling of the collagen on the activated microspheres. Furthermore, the effects of coating conditions, activation methods of collagen, sources of collagen and DEAE dosage on the microcarrier cell culture were examined. The results show that the collagen layer of charge-collagen double acting microcarrier can be controlled to 5~50 mm. After culture for 2 h, the cells attached to about 4×104 cells/mL, the attachment rate is between Cytodex 1 and Cydodex 3. The growth rate is faster than Cydodex 1 after 96 h, and cell number is more than Cytodex 1 and Cydodex 3 in final 144 h, reaching 1.59×106 cells/mL.
汪少久 王启宝 李娟 马光辉 周炜清. 电荷-胶原蛋白双作用微载体的制备和性能调控[J]. , 2015, 15(2): 295-300.
WANG Shao-jiu WANG Qi-bao LI Juan MA Guang-hui ZHOU Wei-qing;. Preparation and Property Regulation of Charge-Collagen Double Acting Microcarrier. , 2015, 15(2): 295-300.
2015, Vol. 15 
