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过程工程学报 ›› 2018, Vol. 18 ›› Issue (4): 866-871.DOI: 10.12034/j.issn.1009-606X.217354

• 环境与能源 • 上一篇    下一篇

BAF工艺预处理含微囊藻毒素微污染水源的微生物特征

张文艺1*, 王逸超1, 蔡庆庆1,2   

  1. 1. 常州大学环境与安全工程学院,江苏 常州 213164;
    2. 江苏龙环环境科技有限公司,江苏 常州 213127
  • 收稿日期:2017-10-10 修回日期:2017-12-19 出版日期:2018-08-22 发布日期:2018-08-15
  • 通讯作者: 张文艺 zhangwenyi888@sina.com
  • 基金资助:
    国家自然科学基金青年项目;“十三五”国家水体污染控制与治理科技重大专项

Microbial characterization of micro polluted source water containing microcystin pretreatment with biological aerated filter

Wenyi ZHANG1*, Yichao WANG1, Qingqing CAI1,2   

  1. 1. School of Environmental & Safety Engineering, Changzhou University, Changzhou, Jiangsu 213164, China
    2. Jiangsu Longhuan Environmental Science Co., Ltd., Changzhou, Jiangsu 213127, China
  • Received:2017-10-10 Revised:2017-12-19 Online:2018-08-22 Published:2018-08-15

摘要: 以曝气生物滤池载体表面的生物膜为研究对象,通过镜检、磷脂脂肪酸(PLFA)测定、高通量454测序等手段研究了生物膜降解含氮有机物和微囊藻毒素-亮氨酸(MC-LR)的微生物特征. 结果表明,低碳源的微污染水源中水生物膜的微生物群落极为丰富,形成了由好氧细菌组成的微生物群落,运行前2周有6大门类17大种属,3?4周增加到14大门类43大种属,还有线虫、草履虫、水蚤等原生动物;优势菌属有球衣菌属Sphaerotilus (2.41%?24.58%)、气单胞属Aeromonas (4.16%?12.59%)、黄杆菌属Cloacibacterium (1.85%?12.39%)、水杆菌属Aquabacterium (1.53%?6.76%)、噬氢菌属Hydrogenophaga (1.12%?5.9%)、嗜甲基菌属Methyloversatilis (0.53%?1.52%)、红杆菌属Rhodobacter (0.09%?1.39%)等. 此外,投加0.16% T1菌剂芽孢杆菌后其含量增至1.97%,表明投加T1降解菌后芽孢杆菌迅速生长并成为优势种群,可强化降解MC-LR.

关键词: 水源水, 曝气生物滤池, 高通量454, 微生物

Abstract: The microbial characteristics of biodegradation of organic compounds containing nitrogen and microcystin LR (MC-LR) was researched with the biofilm of biological aerated filter (BAF) reactor surface as the research object, by the means of microscopic examination, phospholipid fatty acid (PLFA), high-throughput sequencing and others. The results showed that the microbial community on the biofilm of BAF was extremely rich in the micro-polluted source water of low carbon source, formed the microbial community consists of aerobic bacteria, sulfate reducing bacteria and other bacteria. In the first 2 weeks, the microflora on the biofilm had 17 major genera and 6 species. In the 3 to 4 weeks, the microflora will increase to 43 major genera and 14 species. There also were protists such as nematodes, paramecium and daphnia. The dominant species were Sphaerotilus (2.41%?24.58%), Aeromonas (4.16%?12.59%), Cloacibacterium (1.85%?12.39%), Aquabacterium (1.53%?6.76%), Hydrogenophaga (1.12%?5.9%), Methyloversatilis (0.53%?1.52%), Rhodobacter (0.09%?1.39%), etc. In addition, the content of bacillus increased from the initial 0.16% to 1.97% after the addition of T1 degrading bacteria, which showed that bacillus grew rapidly and became dominant population after adding T1 degrading bacteria, thus enhancing the degradation of MC-LR.

Key words: Drinking water sources, Biological aerated filter, High-throughput sequencing 454