Abstract：Plasmid stability and the activity of b-lactamase were examined in the factorial fed-batch cultures of E.coli HB101(pBR322) in a fermentor. No loss of plasmid pBR322 was observed when temperature shifted from 33 oC to 39 oC, pH from 6.4 to 7.2, dissolved oxygen (DO) from 40% to 80%, the feed rate from 5.4 g/h to 10.8 g/h under the condition of density up to 27.3 g/L and the specific growth rate up to 0.73 h-1. The specific activity of b-lactamase, however, decreased in high cell density cultures.
喻国策;焦瑞身;王骥程;王树青. 大肠杆菌E.coli HB101(pBR322) 高密度培养过程质粒的稳定性[J]. , 2001, 1(2): 0-0.
YU Guo-ce; JIAO Rui-shen; WANG Ji-cheng; WANG Shu-qing. Plasmid Stability in High Cell Density Cultures of E.coli HB101(pBR322). , 2001, 1(2): 0-0.