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›› 2001, Vol. 1 ›› Issue (2): 0-0.

• 2 •    

大肠杆菌E.coli HB101(pBR322) 高密度培养过程质粒的稳定性

喻国策,焦瑞身,王骥程,王树青   

  1. 1. 中科院化工冶金研究所生化工程国家重点实验室,北京 100080;2. 中科院上海植物生理研究所,上海 200032;3. 浙江大学工业过程控制研究所,浙江 杭州 310027
  • 出版日期:2001-04-20 发布日期:2001-04-20

Plasmid Stability in High Cell Density Cultures of E.coli HB101(pBR322)

YU Guo-ce1, JIAO Rui-shen2, WANG Ji-cheng3, WANG Shu-qing3   

  1. 1. State Key Lab. Biochem. Eng., Inst. Chem. Metall., CAS, Beijing 100080, China; 2. Shanghai Inst. Plant Physiol., CAS, Shanghai 200032, China; 3. Inst. Ind. Process Control, Zhejiang Univ., Hangzhou, Zhejiang 310027, China
  • Online:2001-04-20 Published:2001-04-20

摘要: 通过正交实验考察了大肠杆菌E.coli HB101(pBR322)高密度培养过程中,不同培养条件对质粒的稳定性和β-内酰胺酶活力的影响. 结果表明,当温度在33~39℃、pH为6.4~7.2、DO为 40%~80%、 补料速率在5.4~10.8 g/h范围内变化,以及细胞密度达到27.3 g/L、比生长速率达到0.73 h-1时,质粒没有发生丢失现象,但在培养过程中β-内酰胺酶的活力降低.

关键词: 大肠杆菌E.coli HB101(pBR322), 高密度培养, 质粒稳定性, β-内酰胺酶

Abstract: Plasmid stability and the activity of b-lactamase were examined in the factorial fed-batch cultures of E.coli HB101(pBR322) in a fermentor. No loss of plasmid pBR322 was observed when temperature shifted from 33 oC to 39 oC, pH from 6.4 to 7.2, dissolved oxygen (DO) from 40% to 80%, the feed rate from 5.4 g/h to 10.8 g/h under the condition of density up to 27.3 g/L and the specific growth rate up to 0.73 h-1. The specific activity of b-lactamase, however, decreased in high cell density cultures.

Key words: E.coli HB101(pBR322), high cell density culture, plasmid stability, b-lactamase

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