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›› 2005, Vol. 5 ›› Issue (3): 260-264.

• 反应与分离 • 上一篇    下一篇

竞争性抑制剂对反胶团萃取过程中α胰凝乳蛋白酶活力的稳定作用

刘俊果,沈睿,邢建民,阳承利,刘会洲   

  1. 中国科学院过程工程研究所
  • 出版日期:2005-06-20 发布日期:2005-06-20

Enhanced Activity Recovery of α-Chymotrypsin during Reverse Micelles Extraction by Addition of Competitive Inhibitor

LIU Jun-guo,SHEN Rui,XING Jian-min,YANG Cheng-li,LIU Hui-zhou   

  1. Lab. Sep. Sci. & Eng., Institute of Process Engineering, Chinese Academy of Sciences
  • Online:2005-06-20 Published:2005-06-20

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摘要： 研究了竞争性抑制剂苯基硼酸对a胰凝乳蛋白酶反胶团萃取过程的影响及对酶活力的稳定作用. 研究发现，向AOT/isooctane 反胶团中添加一定量的苯基硼酸，萃取率会略有增加，反萃取率则无显著变化. 酶活力的回收率显著提高，酶活力对萃取和反萃时间的耐受性显著增强. 苯基硼酸和a胰凝乳蛋白酶之间的特异性亲和作用是产生这种影响的原因.

关键词: 反胶团萃取, 竞争性抑制剂, α胰凝乳蛋白酶, 苯基硼酸

Abstract: The effect of phenylboronic acid, a competitive inhibitor, on the extraction process and activity recovery of a-chymotrypsin by AOT[sodium 1,4-bis(2-ethylhexyl) sulfusuccinate] reverse micelles was studied. A moderate increase of extraction and in significant change of stripping were found when 2 mmol/L phenylboronic acid was added into the AOT reverse micelles system. The profound effect was that the activity recovery was greatly enhanced. The stability of enzyme activity was time dependent: the addition of phenylboronic acid induced enhanced activity recovery in a prolonged contact time during extraction and stripping. The specific binding of phenylboronic acid to chymotrypsin was considered to account for the stabilizing effect of enzyme activity during reverse micelles extraction.

Key words: reverse micelles extraction, competitive inhibitor, α-chymotrypsin, phenylboronic acid