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›› 2007, Vol. 7 ›› Issue (2): 385-389.

• 生化工程专栏 • 上一篇    下一篇

电场对黄孢原毛平革菌生长、细胞通透性及其胞外酶反应的影响

王福远,苗长春,韩慧龙,金文标,刘铮   

  1. 清华大学化工系
  • 出版日期:2007-04-20 发布日期:2007-04-20

Effect of Electric Field on Cell Growth, Permeability and Extracellular Enzymatic Reactions of Phanerochaete chrysosporium

MIAO Fu-yuan,MIAO Chang-chun,HAN Hui-long,JIN Wen-biao,LIU Zheng   

  1. Department of Chemmical Engineering, Tsinghua Univisity
  • Online:2007-04-20 Published:2007-04-20

摘要: 研究了交、直流电场对黄孢原毛平革菌Phanerochaete chrysosporuim的生长、细胞通透性及其所分泌的漆酶、木质素过氧化物酶及锰型过氧化物酶活力的影响. 结果表明,施加50 Hz, 50 mA交流电可显著地促进Phanerochaete chrysosporium的生长,经过10 d的培养,其干菌重为对照组的1.72倍;施加交、直流电都能够使细胞的通透性增强;施加交、直流电场在短时间内可显著提高3种胞外酶的活力,而时间过长则导致活力降低,电流强度为10 mA时的适宜加电时间为10 h.

关键词: 电刺激, 黄孢原毛平革菌, 漆酶, 木质素过氧化物酶, 锰型过氧化物酶

Abstract: The effect of electric field on the cell growth and permeability of Phanerochaete chrysosporium, and extracellular enzymatic activity of laccase, lignin peroxidase and manganese peroxidase, squeezed extracellularly out of Phanerochaete chrysosporium, was experimentally investigated, respectively. The application of 50 Hz alternating current of 50 mA enhanced the growth of Phanerochaete chrysosporium, giving a 1.72 fold increase in cell dry weight after 10 d culture. The cell permeability was also increased when exposed to either DC or AC. The enzymatic activity of laccase, lignin peroxidase and manganese peroxidase was firstly increased and then reduced when exposed to DC, and the suitable operation time was 10 h for DC of 10 mA.

Key words: electrostimulation, Phanerochaete chrysosporium, laccase, lignin peroxidase, manganese peroxidase