关键词: 代谢, 麦拓莱霉素, 藤黄灰链霉菌099, 脂肪酸酯

Abstract: To establish the relationship between the biosynthetic pathway of a primary metabolite fatty acid ester and that of a novel macrolide maituolaimycin, a multi-separation chromatographic method was used to separate and purify the fatty acid ester from the fermentation broth of Streptomyces regnesis 099. And the structure of this fatty acid ester was identified using NMR and ESI-MS. A rational model was built in regard to the relationship of the two mentioned above and was validated by the fermentation experiments. The results showed that both polyketide biosynthetic pathway and fatty acid ester biosynthetic pathway existed in the strain, which is helpful to the optimization of fermentation technology.

Key words: metabolism, maituolaimycin, Streptomyces regensis 099, fatty acid ester