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›› 2009, Vol. 9 ›› Issue (1): 101-106.

• 生化工程专栏 • 上一篇    下一篇

荧光磁性双功能Fe3O4@PHEMA-Tb微球的制备及其蛋白固定化

孟繁宗 王东来 翟玉春   

  1. 东北大学材料与冶金学院 鞍山师范学院化学系 东北大学 材料与冶金学院
  • 收稿日期:2008-09-05 修回日期:2008-11-20 出版日期:2009-02-20 发布日期:2009-02-20
  • 通讯作者: 王东来

Preparation and Protein Immobilization of Fluorescent-Magnetic Bifunctional Fe3O4@PHEMA-Tb Microspheres

MENG Fan-zong LIU Dong-lai ZHAI Yu-chun   

  1. School of Meterials & Metallurgy, Northeastern University Department of Chemistry, Anshan normal university College of Material and Metallurgy, Northeastern University
  • Received:2008-09-05 Revised:2008-11-20 Online:2009-02-20 Published:2009-02-20
  • Contact: LIU Dong-lai

摘要: 以甲基丙烯酸-2-羟基乙酯为单体,N,N¢-亚甲基双丙烯酰胺为交联剂,采用光化学方法在Fe3O4磁性液体中制备了磁性聚甲基丙烯酸-2-羟基乙酯微球,合成了含有稀土元素Tb的荧光磁性高分子微球,以牛血清白蛋白为模型对微球固定释放蛋白能力进行了研究,用VSM, PCS, FT-IR, TG-DTA, SEM, FS, UV-Vis等技术对微球的性能进行了表征. 结果表明,荧光磁性高分子微球粒径为29.6 nm,比饱和磁化强度为40.1 emu/g,变异系数为3.7%,具有超顺磁性荧光性,分散性好,呈圆球形,对蛋白的装载率和包封率分别为6.5%和74.7%,pH越低蛋白释放率越高.

关键词: 荧光磁性高分子微球, Fe3O4磁性液体, 甲基丙烯酸-2-羟基乙酯, 稀土元素, 光化学合成, 蛋白固定化

Abstract: Taking 2-hydroxyethyl methacrylate as the monomer, N,N¢-methyl radical double acrylics amide as cross linking agent, magnetic nanometer particles including hydroxyl were prepared by photochemical synthesis in the magnetic fluid Fe3O4. Fluorescent magnetic polymer microspheres (FMPMs) were synthesized by combining Tb, and immobilized BSA on the suface of microspheres at the same time. The magnetism, average size, structure, morphology, fluorescence and loading properties of FMPMs were characterized by VSM, PCS, FT-IR, TG, SEM, FS and UV-Vis techniques. The results indicated that FMPMs had an average diameter of 29.6 nm, saturated magnetization ratio of 40.1 emu/g, variational coefficient of 3.7%, superparamagnetism, well fluorescence and dispersion and sphericity with the ratio of loading with BSA of 6.5%, and ratio of coating with BSA of 74.7%. Moreover, their protein release rate decreased with pH reduction.

Key words: fluorescent magnetic polymer microspheres, Fe3O4 magnetic fluid, 2-hydroxyethyl methacrylate, rare-earth element, photochemical synthesis, protein immobilization

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