Abstract：Taking 2-hydroxyethyl methacrylate as the monomer, N,N￠-methyl radical double acrylics amide as cross linking agent, magnetic nanometer particles including hydroxyl were prepared by photochemical synthesis in the magnetic fluid Fe3O4. Fluorescent magnetic polymer microspheres (FMPMs) were synthesized by combining Tb, and immobilized BSA on the suface of microspheres at the same time. The magnetism, average size, structure, morphology, fluorescence and loading properties of FMPMs were characterized by VSM, PCS, FT-IR, TG, SEM, FS and UV-Vis techniques. The results indicated that FMPMs had an average diameter of 29.6 nm, saturated magnetization ratio of 40.1 emu/g, variational coefficient of 3.7%, superparamagnetism, well fluorescence and dispersion and sphericity with the ratio of loading with BSA of 6.5%, and ratio of coating with BSA of 74.7%. Moreover, their protein release rate decreased with pH reduction.