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›› 2009, Vol. 9 ›› Issue (1): 128-132.

• 生化工程专栏 • 上一篇    下一篇

原位转移技术用于酵母合成2-苯乙醇

关昂 王航 孟春 石贤爱 郭养浩   

  1. 福州大学药物生物技术与工程研究所 福州大学生物科学与工程学院 福州大学生物科学与工程学院 福州大学生物科学与工程学院 福州大学生物科学与工程学院
  • 收稿日期:2008-09-12 修回日期:2008-12-22 出版日期:2009-02-20 发布日期:2009-02-20
  • 通讯作者: 郭养浩

Biosynthesis of 2-Phenylethanol by Saccharomyces cerevisiae sp. Strain with ISPR Technology

GUAN Ang WANG Hang MENG Chun SHI Xian-ai GUO Yang-hao   

  1. College of biological science and technologe , Fuzhou University College of biological science and technology, Fuzhou University College of biological science and technologe , Fuzhou University College of biological science and technologe , Fuzhou University College of biological science and technologe , Fuzhou University
  • Received:2008-09-12 Revised:2008-12-22 Online:2009-02-20 Published:2009-02-20
  • Contact: GUO Yang-hao

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被引次数

25

摘要： 研究了酵母转化L-苯丙氨酸(Phe)合成2-苯乙醇(Pea)的常规和带原位转移的补料分批培养过程特性. 在常规培养中，以优化补料策略将糖浓度控制在0.1~0.3 g/L，使副产物乙醇浓度小于1%，而Pea的最高终浓度仅为3.85 g/L，因产物抑制效应无法获得更高浓度. 采用大孔树脂FD0816作为原位转移产物的介质，Pea最终总浓度达到12.80 g/L，平均生成速率为0.38 g/(L×h)，比未添加树脂的培养体系分别提高了232%和35.7%. 采用乙醇溶液对发酵用的树脂进行动态洗脱，Pea洗脱率达到95%以上，洗脱液中Pea浓度达到60 g/L.

关键词: 生物合成, 2-苯乙醇, 产物原位转移, 大孔树脂

Abstract: The bioconversion characteristics of L-phenylalanine (Phe) to 2-phenylethanol (Pea) by the yeast Saccharomyces cerevisiae in normal fed-batch culture and ISPR-fed-batch culture were studied respectively. In the normal fed-batch culture, the glucose concentration was controlled in 0.1~0.3 g/L and then the byproduct ethanol concentration was lower than 1%, but the final Pea concentration was lower than 3.85 g/L. Higher Pea concentration could not be attained due to the product inhibition. Macroporous resin FD0816 was chosen as a product in situ extraction material, the final Pea concentration reached 12.80 g/L, and Pea average production rate 0.38 g/(L×h), which were improved by 232% and 35.7% respectively as compared with that without resin addition. The resin used in fermentation was dynamically eluted by ethanol solution, the stripping rate was more than 95% and Pea concentration in eluent achieved 60 g/L.

Key words: biosynthesis, 2-phenylethanol, in situ product removal, macroporous resin

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