放线菌酮对米根霉积累L-苹果酸代谢途径的调控作用

›› 2009, Vol. 9 ›› Issue (1): 153-156.

放线菌酮对米根霉积累L-苹果酸代谢途径的调控作用

何皓徐晴李霜张凯黄和

南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院

收稿日期:2008-10-13 修回日期:2008-12-02 出版日期:2009-02-20 发布日期:2009-02-20
通讯作者: 黄和

Effect of Cycloheximide on Regulation of Metabolic Pathway for L-Malic Acid Accumulation by Rhizopus oryzae

HE Hao LI Shuang LI Shuang ZHANG Kai HUANG He

College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology College of Life Science and Pharmaceutical Engineering. Nanjing University of Technology

Received:2008-10-13 Revised:2008-12-02 Online:2009-02-20 Published:2009-02-20
Contact: HUANG He

摘要/Abstract

摘要： 实验考察了富马酸酶活抑制剂放线菌酮对米根霉发酵产L-苹果酸和富马酸的影响. 结果表明，发酵16 h加入浓度15 mg/L的放线菌酮使米根霉富马酸酶胞质同功酶比活力峰值降低48.7%，而苹果酸脱氢酶胞质同功酶活力却未有明显改变，生成的富马酸比对照样减少37.1%，而L-苹果酸积累量提高54.6%，达到21.7 g/L. 表明通过对米根霉富马酸酶胞质同功酶的抑制作用能减弱米根霉积累富马酸代谢途径中由L-苹果酸向富马酸的转化，从而促进米根霉积累L-苹果酸.

关键词: L-苹果酸, 米根霉, 放线菌酮, 富马酸酶, 富马酸

Abstract: The effects of fumarase inhibitor cycloheximide on fermentation of L-malic acid and fumaric acid by Rhizopus oryzae ME-M15 were investigated. The results showed that the addition of cycloheximide (15 mg/L) during the acid production stage after 16 h incubation greatly affected the metabolism of Rhizopus oryzae ME-M15. The activity of cytosolic isoenzyme of fumarase was decreased markedly by 48.7%, but the activity of NAD-malate dehydrogenase in the cytosol fraction did not change significantly in the presence of cycloheximide. Fumaric acid production was severely decreased in the presence of cycloheximide with 37.1% in comparison with the control mycelium. In contrast to the inhibitory effect of fumaric acid fermentation, the addition of cycloheximide caused the increase of L-malic acid accumulation to 21.7 g/L, which was 54.6% higher than that of the control. The analyses revealed that the inhibition of fumarase isoenzyme in the cytosol fraction could reduce the metabolic flux from L-malic acid to fumaric acid in the pyruvate carboxylation pathway (cytosolic pathway), promoting the L-malic acid accumulation by Rhizopus oryzae ME-M15.

Key words: L-malic acid, Rhizopus oryzae, cycloheximide, fumarase, fumaric acid

中图分类号:

TQ921.7
Q93

何皓徐晴李霜张凯黄和. 放线菌酮对米根霉积累L-苹果酸代谢途径的调控作用[J]. , 2009, 9(1): 153-156.

HE Hao LI Shuang LI Shuang ZHANG Kai HUANG He. Effect of Cycloheximide on Regulation of Metabolic Pathway for L-Malic Acid Accumulation by Rhizopus oryzae[J]. , 2009, 9(1): 153-156.

[1]	胡嘉欢张谷涵付永前朱婷婷. 米根霉一步发酵法高效积累L-乳酸的策略[J]. 过程工程学报, 2017, 17(2): 375-381.
[2]	吴王杰洪璐孙小龙付永前. 钙离子对米根霉菌体形态及L-乳酸合成的影响[J]. , 2014, 14(4): 655-659.
[3]	张亚丹万永军陈丽燕李佩蓓付永前. 米根霉磁性生物吸附剂的制备及其对刚果红的吸附[J]. , 2012, 12(6): 1014-1019.
[4]	付永前黄和李霜赵朋朋何皓韦萍. 碱性树脂吸附富马酸及富马酸与葡萄糖的分离[J]. , 2009, 9(1): 138-142.
[5]	刘宁李霜何皓吴华黄和嵇松杨. 少根根霉利用木糖和葡萄糖分步发酵制备富马酸[J]. , 2008, 8(4): 794-797.