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›› 2007, Vol. 7 ›› Issue (1): 133-136.

• 生化工程专栏 • 上一篇    下一篇

藏红花胚性愈伤组织的发生及其调控

陈书安,王晓东,赵兵,王玉春   

  1. 中国科学院过程工程研究所生化工程国家重点实验室
  • 出版日期:2007-02-20 发布日期:2007-02-20

Induction and Regulation of Crocus sativus L. Embryogenic Callus

CHEN Shu-an,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun   

  1. State Key Laboratory Of Biochemical Engineering, Institute Of Process Engineering, Chinese Academy Of Sciences
  • Online:2007-02-20 Published:2007-02-20

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被引次数

14

摘要： 为提高藏红花胚性愈伤组织的繁殖系数和出芽率，促进其生长和分化，以建立藏红花离体快繁体系，解决藏红花资源短缺问题，研究了藏红花胚性愈伤组织的发生及其调控. 结果表明，获得的藏红花球茎1细胞系具有良好的胚性愈伤组织发生能力. 胚性愈伤组织生长的优化条件为：在添加3.0 mg/L 6-BA, 0.25 mg/L NAA和400 mg/L CH的B5固体培养基上，22℃下全时暗培养25 d，繁殖系数达到9 g/g. 胚性愈伤组织出芽的优化条件为：在添加3.0 mg/L 6-BA, 0.25 mg/L NAA和400 mg/L CH的1/2 B5固体培养基上，在22℃及光照强度31.74 mmol/(m2×s)条件下，每天光照10 h，暗培养14 h，培养45 d出芽率达到44.7%，高于国外报道的20%.

关键词: 藏红花, 胚性愈伤组织, 快繁体系, 出芽能力

Abstract: The induction and regulation of embryogenic callus were investigated to promote the growth and shoot induction rate for micropropagation of Crocus sativus L. The embryogenic callus line was obtained easily from one corm callus line (corm 1). After 25 d, the maximum proliferation rate of callus reached 8.9 g/g under the optimized condition for cell growth when the callus was cultured on B5 medium supplemented with 400 mg/L CH, 0.25 mg/L NAA and 3.0 mg/L 6-BA at 22℃ in darkness. After 45 d, the maximum shoot induction rate of callus reached 45% under the optimized condition for shoot induction when the callus was cultured on 1/2 B5 medium supplemented with 400 mg/L CH, 0.25 mg/L NAA and 3.0 mg/L 6-BA at 22℃ under 16 h light with the intensity of 31.74 mmol/(m2×s) per day, while the highest shoot induction rate of Crocus sativus L. callus was reported only about 20% abroad.

Key words: Crocus sativus L, embryogenic callus, micropropagation, shoot induction rate