Abstract：The relationship between the starvation culture and relative cell viability in the growth phase and their effects on the expression of recombinant human IFNb-HSA fusion protein in Pichia pastoris KM71/IH were observed in 5 L fermentor experiments. The results showed that the starvation culture in which glycerol as the solo carbon source was exhausted resulted in the decrease of relative cell viability and expression of recombinant hIFNb-HSA fusion protein. The strategy of mannitol addition in the transition stage not only eliminated the inhibition effect on methanol induction by residual glycerol, but also kept higher relative cell viability (>97%) and then improved the yield of human IFNb-HSA fusion protein. The maximum yield and maximum hIFNb-HSA productivity reached 37.3 mg/L and 0.78 mg/(L×h) respectively, which were 92.3% and 44.4% higher than those in the control.
张宏斌;窦文芳;许泓瑜;金坚;李华钟;许正宏. 提高重组毕赤酵母表达hIFNb-HSA的碳源控制策略[J]. , 2007, 7(6): 1181-1186.
ZHANG Hong-bin;DOU Wen-fang;XU Hong-yu;JIN Jian;LI Hua-zhong;XU Zheng-hong. A Carbon-controlling Strategy to Enhancement of the Expression for Human IFNb-HSA Fusion Protein with Pichia pastoris. , 2007, 7(6): 1181-1186.