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›› 2001, Vol. 1 ›› Issue (4): 0-0.

• 4 •    

抗生素AGPM摇瓶发酵条件的正交实验

石炳兴, 赵红, 刘喜朋, 元英进, 胡宗定   

  1. 天津大学生化工程系, 天津 300072
  • 出版日期:2001-08-20 发布日期:2001-08-20

Improvement of the Fermentation Productivity of a New Antibiotic AGPM by Orthogonal Design Experiment

SHI Bing-xing, ZHAO Hong, LIU Xi-peng, YUAN Ying-jin, HU Zong-ding   

  1. Department of Biochemical Engineering, Tianjin University, Tianjin 300072, China
  • Online:2001-08-20 Published:2001-08-20

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被引次数

103

摘要： 采用正交实验设计考察了培养基组成对新型抗生素AGPM发酵的影响，改进后在培养基组成为(g/L)：葡萄糖5, 玉米淀粉40, 黄豆饼粉16, 玉米浆2 ml, K2HPO4 1.0, MgSO4×7H2O 0.5, NaCl 0.5, 淀粉酶0.05及pH 5.5的条件下，单位发酵液的活性提高了18.9倍；同时表明较高的碳氮比对抗生素AGPM的合成有利.

关键词: 抗生素, 培养基, 链霉菌, 正交实验

Abstract: The effects of medium composition on the activity of a new antibiotic APGM was studied by orthogonal design experiment. It seems that nitrogen source presented the most significant effect on the production of AGPM and that higher ratio of carbon to nitrogen was beneficial. It was concluded that the fermentation activity was increased by 18.9 times to 1562.2 u/ml under the optimal conditions as the medium was composed of glucose 5 g/L, corn starch 40 g/L, soybean meal 16 g/L, corn steep liqour 2 ml, K2HPO4 1.0 g/L, MgSO4·7H2O 0.5 g/L, NaCl 0.5 g/L and amylase 0.05 g/L.

Key words: antibiotic, medium, Streptomyces, orthogonal design

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