关键词: animal cell culture, metabolism, kinetics, glucose, maintenance energy

Abstract: The kinetic model of glucose metabolism was established and successfully applied to batch cultures of rCHO and rBHK cells. It was found that a large amount of glucose was utilized for cell maintenance, and the overwhelming majority of maintenance energy from glucose was by its anaerobic metabolism in both rBHK and rCHO cell cultures. The overall maintenance coefficients from aerobic metabolism were 1.9′10-13 mmol/(cell·h) for rCHO cells and 7′10-13 mmol/(cell·h) for rBHK cells. In addition, all GO/T and EO/T gradually increased with the same trend as the cell growth in the culture of both rCHO and rBHK cells. The overall molecule yield coefficients of lactate to glucose were 1.61 for rCHO cells and 1.38 for rBHK cells. The yield coefficients of cell to glucose were 4.5′108 cells/mmol for rCHO cells and 1.9 ′108 cells/mmol for rBHK cells, respectively.

Key words: animal cell culture, metabolism, kinetics, glucose, maintenance energy