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›› 2011, Vol. 11 ›› Issue (3): 487-491.

• 生化工程专栏 • 上一篇    下一篇

壳聚糖微球的自发荧光机理

宋春艳 王连艳 吴有斌 张竟 马光辉 张栩   

  1. 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 北京化工大学生命科学学院
  • 收稿日期:2011-04-13 修回日期:2011-05-11 出版日期:2011-06-20 发布日期:2011-06-20
  • 通讯作者: 宋春艳

Auto-fluorescent Mechanism of Chitosan Microspheres Crosslinked with Glutaraldehyde

SONG Chun-yan WANG Lian-yan, WU You-bin, ZHANG Jing MA Guang-hui ZHANG Xu   

  1. State Key Lab. Biochem. Eng., Institute of Process Engineering, Chinese Academy of Sciencses . State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences . State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences . State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences College of Life Science and Technology, Beijing University of Chemical Technology
  • Received:2011-04-13 Revised:2011-05-11 Online:2011-06-20 Published:2011-06-20
  • Contact: SONG Chun-yan

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摘要： 为验证壳聚糖的自发荧光机理，以顺-2-甲基-2-丁烯醛和2-氨基-2-甲基丙烷为原料，在-14℃无水无氧条件下合成了具有?C==N?C==C?结构单元的产物，通过傅立叶红外光谱和液质联用技术进行结构确认，并对合成产物进行了荧光光谱扫描和激光共聚焦光谱扫描分析. 结果表明，合成的物质在488 nm激发下，于510~540和570~600 nm波段采集到了稳定的荧光，与壳聚糖荧光微球的荧光波长一致. 据此可推断壳聚糖微球的自发荧光主要源于?C==N? C==C?共轭结构单元.

关键词: 壳聚糖微球, 自发荧光, 机理, 合成, 共轭结构

Abstract: A compound containing ?C==N?C==C? was synthesized by 2-methyl-2-crotonaldehyde and 2-amino-2-methyl propane. Its structure was confirmed by infrared spectroscopy and liquid chromatography-mass spectroscopy. And the results showed that a relatively pure compound was obtained. Furthermore, its fluorescent property was analyzed by fluorescence spectroscopy and laser scanning confocal microscope, which showed the same fluorescent property with chitosan microspheres. Therefore, the conjugating structure of ?C==N?C==C? was mainly attributed to auto-fluorescence of chitosan microspheres crosslinked with glutaraldehyde.

Key words: chitosan microspheres, auto-fluorescence, mechanism, synthesis, conjugating structure

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