Study on Interaction of Ce3+ and Bovine Serum Albumin
WU Jin-xiu LI Mei SONG Yu-min LIU Zhao-gang HU Yan-hong WANG Mi-tang
College of Rare earth, Inner Mongolia University of Science and Technology Inner Mongolia Science and Technology University Material institute College of Chemistry and Chemical Engineering, Northwest Normal University Inner Mongolia Science and Technology University Material institute Inner Mongolia Science and Technology University Material institute Inner Mongolia Science and Technology University Material institute
Abstract：In physiological condition, the binding reaction of Ce3+ and Ce2(CO3)3 with bovine serum albumin (BSA) was studied by fluorescence and UV spectra. The fluorescent mechanism of Ce3+ and Ce2(CO3)3 with BSA was examined, and the combination constant (KA) and thermodynamic parameters (DH, DG and DS) of Ce3+, Ce2(CO3)3 and BSA at different temperatures calculated, and the reaction type of Ce3+ and Ce2(CO3)3 with BSA determined. From the result of DH<0, ΔS>0, It can be judged that Ce3+, Ce2(CO3)3 and BSA had mainly electrostatic attraction. The results showed that Ce3+ and Ce2(CO3)3 reacted with BSA to form new compound systems under nCe3+:nBSA=1.8:1 and nCe2(CO3)3:nBSA=2.6:1. The quenching belonged to static fluorescence quenching caused by non-radiation energy transfer.
吴锦绣 李梅 宋玉民 柳召刚 胡艳宏 王觅堂. Ce3+与牛血清白蛋白的相互作用的研究[J]. , 2010, 10(6): 1126-1131.
WU Jin-xiu LI Mei SONG Yu-min LIU Zhao-gang HU Yan-hong WANG Mi-tang. Study on Interaction of Ce3+ and Bovine Serum Albumin. , 2010, 10(6): 1126-1131.
2010, Vol. 10 
