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›› 2010, Vol. 10 ›› Issue (6): 1126-1131.

• 过程与工艺 • 上一篇    下一篇

Ce3+与牛血清白蛋白的相互作用的研究

吴锦绣 李梅 宋玉民 柳召刚 胡艳宏 王觅堂   

  1. 内蒙古科技大学稀土学院 内蒙古科技大学材料与冶金学院 西北师范大学化学化工学院 内蒙古科技大学材料与冶金学院 内蒙古科技大学材料与冶金学院 内蒙古科技大学材料与冶金学院
  • 收稿日期:2011-01-11 修回日期:1900-01-01 出版日期:2010-12-20 发布日期:2010-12-20
  • 通讯作者: 吴锦绣

Study on Interaction of Ce3+ and Bovine Serum Albumin

WU Jin-xiu LI Mei SONG Yu-min LIU Zhao-gang HU Yan-hong WANG Mi-tang   

  1. College of Rare earth, Inner Mongolia University of Science and Technology Inner Mongolia Science and Technology University Material institute College of Chemistry and Chemical Engineering, Northwest Normal University Inner Mongolia Science and Technology University Material institute Inner Mongolia Science and Technology University Material institute Inner Mongolia Science and Technology University Material institute
  • Received:2011-01-11 Revised:1900-01-01 Online:2010-12-20 Published:2010-12-20
  • Contact: WU Jin-xiu

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摘要： 在模拟动物体生理pH值条件下，用荧光光谱法和紫外吸收光谱法研究了稀土离子铈[Ce3+和Ce2(CO3)3]与牛血清白蛋白(BSA)的结合反应. 探讨了Ce3+和Ce2(CO3)3对BSA的荧光猝灭过程机理，并分别计算了不同温度下Ce3+和Ce2(CO3)3与BSA的结合常数(KA)和实际热力学参数(DH，DG和DS)，并判断了Ce3+和Ce2(CO3)3与BSA结合的作用力类型. 由于所求热力学常数都是DH<0，DS>0，由此可判断Ce3+和Ce2(CO3)3与BSA之间的作用力主要为静电引力. 实验结果表明，Ce3+和Ce2(CO3)3与BSA结合形成复合物，分别为：n(Ce3+):n(BSA)=1.8:1, n[Ce2(CO3)3]:n(BSA)=2.6:1. 导致BSA内源性荧光猝灭是由于分子内的非辐射能量转移而引起的静态猝灭.

关键词: Ce3+, 牛血清白蛋白, 荧光光谱, 紫外光谱

Abstract: In physiological condition, the binding reaction of Ce3+ and Ce2(CO3)3 with bovine serum albumin (BSA) was studied by fluorescence and UV spectra. The fluorescent mechanism of Ce3+ and Ce2(CO3)3 with BSA was examined, and the combination constant (KA) and thermodynamic parameters (DH, DG and DS) of Ce3+, Ce2(CO3)3 and BSA at different temperatures calculated, and the reaction type of Ce3+ and Ce2(CO3)3 with BSA determined. From the result of DH<0, ΔS>0, It can be judged that Ce3+, Ce2(CO3)3 and BSA had mainly electrostatic attraction. The results showed that Ce3+ and Ce2(CO3)3 reacted with BSA to form new compound systems under nCe3+:nBSA=1.8:1 and nCe2(CO3)3:nBSA=2.6:1. The quenching belonged to static fluorescence quenching caused by non-radiation energy transfer.

Key words: Ce3+, bovine serum albumin, fluorescence, UV spectrum