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›› 2011, Vol. 11 ›› Issue (6): 1044-1049.

• 生化工程专栏 • 上一篇    下一篇

基于光滑球拟酵母环境适应性的丙酮酸中试条件优化

杨松鑫 王蒙 汪军 刘立明 陈坚   

  1. 江苏省无锡市江南大学生物工程学院 江苏省无锡市江南大学生物工程学院 江苏省无锡市江南大学生物工程学院 江南大学生物工程学院环境生物技术实验室 江南大学工业生物技术教育部重点实验室
  • 收稿日期:2011-08-23 修回日期:2011-11-07 出版日期:2011-12-20 发布日期:2011-12-20
  • 通讯作者: 刘立明

Optimization of Pyruvate Production Conditions Based on Environmental Adaptability of Torulopsis glabrata in a Pilot Plant Scale

YANG Song-xin WANG Meng WANG Jun LI Zhen CHEN Jian,   

  1. State Key Laboratory of Food Science and Technology, Jiangnan University State Key Laboratory of Food Science and Technology, Jiangnan University State Key Laboratory of Food Science and Technology, Jiangnan University Lab of Biotechnology, School of Biotechnology, Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
  • Received:2011-08-23 Revised:2011-11-07 Online:2011-12-20 Published:2011-12-20
  • Contact: LI Zhen

摘要: 在对比研究3和30 L规模发酵罐中T. glabrata生产丙酮酸性能的基础上,在30 L发酵罐中系统优化并研究了氮源、维生素水平及溶氧水平对T. glabrata生产丙酮酸的影响. 结果表明,以7 g/L尿素作为唯一氮源能促进菌体生长,加快葡萄糖消耗和丙酮酸积累;将硫胺素水平由15 mg/L提高至18 mg/L,菌体干重提高12.5%,生产强度提高21.2%;将通气量调节至2.0 L/min时使丙酮酸产量、得率与生产强度分别提高32.8%,11.7%和59%. 优化结果在30 L发酵罐中得到验证,丙酮酸产量、得率和生产强度分别为84.2 g/L, 0.72 g/g与1.40 g/(L×h),比优化前分别提高21.3%, 13.2%和12.9%.

关键词: 光滑球拟酵母, 环境适应性, 丙酮酸, 发酵优化

Abstract: The production conditons of pyruvate at a pilot scale of 30-L fermentor through increasing environmental adaptability of Torulopsis glabrata were optimized. The fermentation parameters of T. glabrata at 3-L and 30-L fermentors were compared and its mechanism was elucidated. Following the mechanism, the nutrient (nitrogen and thiamine concentration) and environmental (dissolved oxygen) conditions were studied and optimized in 30-L fermentor, the optimum conditions were obtained as followed: 7 g/L urea, 18 mg/L thiamine and 2.0 L/min aeration rate. With the conditions, the titer, yield and productivity of pyruvate were achieved at 84.2 g/L, 0.72 g/g and 1.40 g/(L×h), respectively, which were 21.3%, 13.2% and 12.9% higher than the corresponding values of the control.

Key words: Torulopsis glabrata, environmental adaptability, pyruvate, fermentation optimization

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