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›› 2013, Vol. 13 ›› Issue (4): 591-599.

• 反应与分离 • 上一篇    下一篇

Investigation of Three-liquid-phase Extraction Systems for Simultaneous Separation of Emodin and Rhein

杨兴福 梁向峰 杨良嵘 李文松 邓伏礼 王福春 刘会洲   

  1. 中国科学院过程工程研究所 中国科学院过程工程研究所 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中科院过程工程研究所分离科学与工程青年实验室、生化工程国家重点实验室、中科院研究生院 中国科学院过程工程研究所分离工程与工程青年实验室
  • 收稿日期:2013-04-25 修回日期:2013-05-07 出版日期:2013-08-20 发布日期:2013-08-20
  • 通讯作者: 杨兴福

Investigation of Three-liquid-phase Extraction Systems for Simultaneous Separation of Emodin and Rhein

YANG Xing-fu LIANG Xiang-feng YANG Liang-rong LI Wen-song DENG Fu-li WANG Fu-chun LIU Hui-zhou   

  1. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Institute of Process Engineering, Chinese Academy of Sciences
  • Received:2013-04-25 Revised:2013-05-07 Online:2013-08-20 Published:2013-08-20
  • Contact: YANG Xing-fu

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摘要： Several three-liquid-phase extraction systems comprised of organic extractant, polymer and salt aqueous solution were applied to isolate and purify simulated herbal extract containing emodin and rhein. Some influential factors on the partitioning behaviors were studied. The results revealed that extractant type and solution pH value were crucial factors for improving the separation performance. The optimal separation results were obtained at pH 8.00 with methyl isobutyl ketone as the extractant, 10%(w) polyethylene glycol with the molecular weight 4000 Da, and 10%(w) ammonium sulphate. Under the optimized conditions, 95% emodin and 97% rhein were simultaneously extracted into the organic phase and polymer-rich phase from simulated herbal solution with the initial concentration of 100′10-6(w), respectively, and their separation factor of over 10000 was achieved. By adjusting pH value, back extraction rate of both emodin and rhein could be up to about 99%. With the assistance of molecular simulation program XLOGP3 and molecular orbital package, the possible mechanism of extraction was analyzed, and hydrophobic interaction and hydrogen-bonding interaction might be the major driving forces affecting the partitioning behaviors of emodin and rhein.

关键词: three-liquid-phase extraction, partition, purification, back extraction, anthraquinone, emodin, rhein

Abstract: Several three-liquid-phase extraction systems comprised of organic extractant, polymer and salt aqueous solution were applied to isolate and purify simulated herbal extract containing emodin and rhein. Some influential factors on the partitioning behaviors were studied. The results revealed that extractant type and solution pH value were crucial factors for improving the separation performance. The optimal separation results were obtained at pH 8.00 with methyl isobutyl ketone as the extractant, 10%(w) polyethylene glycol with the molecular weight 4000 Da, and 10%(w) ammonium sulphate. Under the optimized conditions, 95% emodin and 97% rhein were simultaneously extracted into the organic phase and polymer-rich phase from simulated herbal solution with the initial concentration of 100′10-6(w), respectively, and their separation factor of over 10000 was achieved. By adjusting pH value, back extraction rate of both emodin and rhein could be up to about 99%. With the assistance of molecular simulation program XLOGP3 and molecular orbital package, the possible mechanism of extraction was analyzed, and hydrophobic interaction and hydrogen-bonding interaction might be the major driving forces affecting the partitioning behaviors of emodin and rhein.

Key words: three-liquid-phase extraction, partition, purification, back extraction, anthraquinone, emodin, rhein

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