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›› 2013, Vol. 13 ›› Issue (4): 693-697.

• 生化工程专栏 • 上一篇    下一篇

基于细胞元素的二形栅藻廉价培养基的改进

万晶晶 薛升长 丛威 张栩   

  1. 北京化工大学生命科学与技术学院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 北京化工大学生命科学学院
  • 收稿日期:2013-04-08 修回日期:2013-05-16 出版日期:2013-08-20 发布日期:2013-08-20
  • 通讯作者: 万晶晶

Improvement on the Cheap Culture Medium for Scenedesmus dimorphus Based on Elemental Concentrations of Cell

WANG JIng-jing XUE Sheng-zhang CONG Wei ZHANG Xu   

  1. (1. College of Life Science and Technology. Beijing University of Chemical Technology State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Lab. Biochem. Eng., Inst. Process Eng., CAS College of Life Science and Technology, Beijing University of Chemical Technology
  • Received:2013-04-08 Revised:2013-05-16 Online:2013-08-20 Published:2013-08-20
  • Contact: WANG JIng-jing

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摘要： 根据微藻细胞营养元素含量对BG11培养基重新设计，改良培养基中主要营养盐浓度为(mg/L): KH2PO4 32, MgSO4·7H2O 32, (NH4)2SO4 22.4, CaCl2·7H2O 8, FeSO4 2.6. 培养实验结果显示，改良培养基和BG11培养基中二形栅藻的初始生长状态几乎相同，最终油脂产量分别为0.407和0.403 g/L，改良培养基的培养成本为BG11培养基的33%~50%.

关键词: 二形栅藻, 元素平衡, 培养基, 油脂产量

Abstract: An elemental balance approach between culture medium and biomass composition is used to redesign BG11 culture medium, the main nutrients in the modified culture medium are KH2PO4 32 mg/L, MgSO4×7H2O 32 mg/L, (NH4)2SO4 22.4 mg/L, CaCl2×7H2O 8 mg/L, and FeSO4 2.6 mg/L. The results show that Scenedesmus dimorphus almost has the same initial growth curve in the modified and BG11 culture media, and the final volumetric yield of lipid is 0.407 and 0.403 g/L, respectively, in the case of reducing the cultivation cost to 33%~50%, Scenedesmus dimorphus in the modified and BG11 culture media almost obtains the same lipid yield.

Key words: Scenedesmus dimorphus, elemental balancing, culture medium, lipid yield

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