Research Progress in L-Phenylacetylcarbinol Production by Biosynthesis
LI Dan LIN Jian-qun LIN Hui-bin LIN jian-qiang QU Yin-bo
State Key Laboratory of Microbial Technology, Shandong Universit State Key Laboratory of Microbial Technology, Shandong Universit Shandong Institute of Traditional Chinese Medicine, State Key Laboratory of Microbial Technology, Shandong Universit State Key Laboratory of Microbial Technology, Shandong Universit
Abstract：The research progress in biotransformation of L-phenylacetylcarbinol (L-PAC), including mechanism of biocatalysis, strain screening, biotransformation system, process parameters and downstream processing, was reviewed. Strains belonging to the genera of Saccharomyces and Candida I with high enzyme activity of pyruvate decarboxylase are widely used in biotransformation of L-PAC from benzaldehyde and pyruvate. The formation of by-products and toxicity of substrate significantly influence the production of L-PAC. By strain screening and breeding, suitable strains with high tolerance to benzaldehyde and effective reduction of byproducts can be obtained. By applying the process optimization methodologies of fed-batch culture, process control and process parameter optimization, utilization of cell or enzyme immobilization and adoption of aqueous and organic two-phase system, higher L-PAC production can be achieved.
李丹 林建群 林慧彬 林建强 曲音波. 生物转化法生产L-苯基乙酰基甲醇的研究进展[J]. , 2009, 9(1): 190-199.
LI Dan LIN Jian-qun LIN Hui-bin LIN jian-qiang QU Yin-bo. Research Progress in L-Phenylacetylcarbinol Production by Biosynthesis. , 2009, 9(1): 190-199.
2009, Vol. 9 
