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›› 2005, Vol. 5 ›› Issue (5): 545-549.

• 生化工程专栏 • 上一篇    下一篇

肝素-琼脂糖凝胶6FF的制备及其在抗凝血酶III分离纯化中的应用

穆成华,姚红娟,马光辉,连宾   

  1. 中国科学院过程工程研究所生化工程国家重点实验室
  • 出版日期:2005-10-20 发布日期:2005-10-20

Preparation of Heparin-Agarose Gel 6FF and Its Application in the Purification of Antithrombin III

MU Cheng-hua,YAO Hong-juan,MA Guang-hui,LIAN Bin   

  1. National Laboratory of Biochemical Engineering, Institute of Process Engineering
  • Online:2005-10-20 Published:2005-10-20

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被引次数

49

摘要： 以自制琼脂糖凝胶6FF为基质，肝素为配基，利用还原胺化方法制备了肝素-琼脂糖凝胶6FF介质. 考察了肝素偶联反应的影响因素，并对其进行了优化，得到肝素配基的含量为2.7 mg/mL. 用所制介质从人血浆中分离出抗凝血酶III，从血浆开始计算的抗凝血酶III活性回收率为31.76%，与商品Heparin-Sepharose CL-6B的分离效果相当.

关键词: 亲和层析, 肝素, 抗凝血酶III

Abstract: The self-prepared bead-formed agarose gel was used as the affinity support, and heparin was employed as the ligand. Heparin was linked covalently to amino-agarose beads, which was prepared by amination of epoxy-agarose beads. The effects of operating conditions on the coupling reaction were investigated and optimized. The amount of heparin immobilized on the synthesized gel was 2.7 mg/mL medium. The synthesized affinity medium was used to isolate antithrombin III from human plasma. The recovery of antithrombin III activity was 31.76%, which was similar to that of Heparin-Sepharose CL-6B from Pharmacia, Sweden.

Key words: affinity chromatography, heparin, antithrombin III