基于生长代谢耦联的1,3-丙二醇发酵过程底物流加控制策略

›› 2012, Vol. 12 ›› Issue (6): 996-1001.

基于生长代谢耦联的1,3-丙二醇发酵过程底物流加控制策略

宋志远滕虎修志龙

大连理工大学生命科学与技术学院大连理工大学生命科学与技术学院大连理工大学生命科学与技术学院

收稿日期:2012-09-07 修回日期:2012-11-15 出版日期:2012-12-20 发布日期:2012-12-20
通讯作者: 宋志远

Coupled-feeding Strategy Based on the Cell Growth and Metabolism during 1,3-Propanediol Fermentation

SONG Zhi-yuan TENG Hu XIU Zhi-long

School of Life Science and Biotechnology, Dalian University of Technology School of Life Science and Biotechnology, Dalian University of Technology School of Life Science and Biotechnology, Dalian University of Technology

Received:2012-09-07 Revised:2012-11-15 Online:2012-12-20 Published:2012-12-20
Contact: SONG Zhi-yuan

摘要/Abstract

摘要： 研究了克雷伯氏杆菌转化甘油生产1,3-丙二醇的底物流加控制策略，其特征是批式流加发酵过程与细胞生长和代谢相耦联. 通过细胞生长动力学分析，建立了对数生长期和稳定期底物消耗与生物量和碱液消耗量之间的函数关系. 2次在线反馈补料发酵实验结果表明，甘油浓度控制在20±2 g/L时，1,3-丙二醇的终浓度分别达80.3和78.8 g/L以上，与手动反馈补料相比，1,3-丙二醇浓度分别提高了25.0和23.5 g/L.

关键词: 克雷伯氏杆菌, 3-丙二醇, 流加控制, 生长代谢

Abstract: A substrate feeding strategy was studied in the fed-batch fermentation of 1,3-propanediol by Klebsiella pneumoniae, which was distinctively coupled with cell growth and metabolism using glycerol as substrate. The functional relationship between substrate consumption, biomass and alkaline consumption was established by analysis of the cell growth kinetics during logarithmic growth and stationary phase, respectively. The fed-batch on-line experimental results showed that the glycerol concentration was controlled at 20±2 g/L, and the final 1,3-propanediol concentration reached 80.3 and 78.8 g/L, which increased 25.0 and 23.5 g/L compared with that of manual feeding-back strategy.

Key words: Klebsiella pneumoniae, 3-propanediol, feeding strategy, growth metabolism

中图分类号:

Q815

宋志远滕虎修志龙. 基于生长代谢耦联的1,3-丙二醇发酵过程底物流加控制策略[J]. , 2012, 12(6): 996-1001.

SONG Zhi-yuan TENG Hu XIU Zhi-long. Coupled-feeding Strategy Based on the Cell Growth and Metabolism during 1,3-Propanediol Fermentation[J]. , 2012, 12(6): 996-1001.

[1]	吴家鑫王凤寰王峥谭天伟刘沛溢. 蔗糖与葡萄糖作辅助碳源对重组Klebsiella pneumoniae发酵生产 1,3-丙二醇的影响[J]. , 2014, 14(4): 660-663.
[2]	李清黄艳娜李志敏叶勤. 重组肺炎克雷伯菌发酵联产3-羟基丙酸和1,3-丙二醇[J]. , 2014, 14(1): 133-138.
[3]	曾宏方柏山瞿引弟. 丙烯醛水合加氢法生产1,3-丙二醇过程模拟与技术经济分析[J]. , 2013, 13(4): 626-632.
[4]	朱晓丽南南修志龙. 以粗甘油为原料高产1,3-丙二醇菌株的等离子体复合诱变[J]. , 2011, 11(6): 1030-1037.
[5]	董晓宇滕虎修志龙. 高产1,3-丙二醇的等离子体诱变菌株选育及发酵动力学分析[J]. , 2011, 11(2): 304-311.
[6]	牟晓佳滕虎孙亚琴修志龙. 甘油发酵生产1,3-丙二醇工艺的技术经济性分析[J]. , 2009, 9(5): 947-952.
[7]	刘国兴王元好孙丽慧戴建英孙亚琴董悦生修志龙. 盾叶薯蓣糖化液发酵生产2,3-丁二醇[J]. , 2009, 9(1): 95-100.
[8]	李爽董晓宇侯英敏修志龙. 大气压冷等离子体诱变产1,3-丙二醇菌株Klebsiella pneumoniae[J]. , 2008, 8(3): 555-560.
[9]	黄志华;张延平;杜晨宇;黄星;曹竹安. 利用氧化还原电位调控基因工程菌株Klebsiella pneumoniae F-1合成1,3-丙二醇[J]. , 2007, 7(5): 1014-1017.
[10]	张延平;刘铭;杜晨宇;沈金玉;曹竹安. 代谢副产物对Klebsiella pneumoniae生长及合成1,3-丙二醇的影响[J]. , 2006, 6(5): 804-808.
[11]	张代佳;高素军;孙亚琴;修志龙. 超滤-醇沉法从发酵液中提取1,3-丙二醇[J]. , 2006, 6(3): 454-457.
[12]	林日辉;刘宏娟;孙燕;郝健;程可可;刘德华. 氧对Klebsiella pneumoniae产1,3-丙二醇代谢的影响[J]. , 2006, 6(1): 96-99.
[13]	王宝光;刘铭;杜晨宇;沈金玉;曹竹安. 微生物法生产1,3-丙二醇过程的代谢工程研究进展[J]. , 2006, 6(1): 144-149.
[14]	杜晨宇;刘铭;饶治;包晓玮;沈金玉;曹竹安. 分段通气对Klebsiella pneumoniae生产1,3-丙二醇关键酶和辅酶的影响[J]. , 2005, 5(5): 540-544.
[15]	程可可;林日辉;刘宏娟;刘德华. 1,3-丙二醇分批发酵动力学模型[J]. , 2005, 5(4): 425-429.