Coupled-feeding Strategy Based on the Cell Growth and Metabolism during 1,3-Propanediol Fermentation
SONG Zhi-yuan TENG Hu XIU Zhi-long
School of Life Science and Biotechnology, Dalian University of Technology School of Life Science and Biotechnology, Dalian University of Technology School of Life Science and Biotechnology, Dalian University of Technology
Abstract：A substrate feeding strategy was studied in the fed-batch fermentation of 1,3-propanediol by Klebsiella pneumoniae, which was distinctively coupled with cell growth and metabolism using glycerol as substrate. The functional relationship between substrate consumption, biomass and alkaline consumption was established by analysis of the cell growth kinetics during logarithmic growth and stationary phase, respectively. The fed-batch on-line experimental results showed that the glycerol concentration was controlled at 20±2 g/L, and the final 1,3-propanediol concentration reached 80.3 and 78.8 g/L, which increased 25.0 and 23.5 g/L compared with that of manual feeding-back strategy.
宋志远 滕虎 修志龙. 基于生长代谢耦联的1,3-丙二醇发酵过程底物流加控制策略[J]. , 2012, 12(6): 996-1001.
SONG Zhi-yuan TENG Hu XIU Zhi-long. Coupled-feeding Strategy Based on the Cell Growth and Metabolism during 1,3-Propanediol Fermentation. , 2012, 12(6): 996-1001.
2012, Vol. 12 
