Screening and Identification of 2-Keto-D-gluconic Acid-producing Strain
NIU Pan-qing YANG Ai-hua YANG Song-xin LI Zhen CHEN Jian,
State Key Laboratory of Food Science and Technology, Jiangnan University State Key Laboratory of Food Science and Technology, Jiangnan University State Key Laboratory of Food Science and Technology, Jiangnan University Lab of Biotechnology, School of Biotechnology, Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
Abstract：A 2-keto-D-gluconic acid (2-KGA) producing strain Serratia sp. FMME043 was isolated from the soil by the method of flat discolor circle with (NH4)2SO4 as nitrogen source. The optimal conditions, glucose 180 g/L, (NH4)2SO4 2.0 g/L and KH2PO4 1 g/L, were obtained through one-factor experimental design. Furthermore, the highest 2-KGA concentration 169.5 g/L and yield 0.87 mol/mol were achieved under the optimum conditions with initial pH 7.0, liquid volume 75 mL in a 750 mL flask, culture temperature 30℃, and shaker rotating speed 200 r/min. These results were also confirmed in a 7 L fermentor.