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›› 2009, Vol. 9 ›› Issue (4): 801-805.

• 生化工程专栏 • 上一篇    下一篇

羰基还原酶与甲酸脱氢酶偶联催化制备(R)-苯基乙二醇

张荣珍 徐岩 耿亚维 王珊珊 孙莹   

  1. 江南大学生物工程学院教育部工业生物技术重点实验室 教育部工业生物技术重点实验室，江南大学生物工程学院 教育部工业生物技术重点实验室，江南大学生物工程学院 江南大学生物工程学院教育部工业生物技术重点实验室
  • 收稿日期:2008-12-04 修回日期:2009-04-07 出版日期:2009-08-20 发布日期:2009-08-20
  • 通讯作者: 徐岩

Preparation of (R)-1-Phenyl-1,2-ethanediol by Biotransformation with a Coupled System of (R)-Specific Carbonyl Reductase and Formate Dehydrogenase

ZHANG Rong-zhen, XU Yan, DI Ya-wei, WANG Shan-shan, SUN Ying,   

  1. Sch.Biotechnology, Jiangnan University Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, SouthenYangtze UniVersity Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, SouthenYangtze UniVersity Sch.Biotechnology, Jiangnan University
  • Received:2008-12-04 Revised:2009-04-07 Online:2009-08-20 Published:2009-08-20
  • Contact: XU Yan,

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摘要： 以重组菌E. coli Rosetta/pETDuet-rcr-fdh全细胞为催化剂，2-羟基苯乙酮和甲酸钠为双底物，研究了生物催化反应体系中温度、pH值、底物2-羟基苯乙酮的初始浓度、反应时间、细胞浓度和状态等对产物(R)-苯基乙二醇生成效率的影响规律. 结果表明，在温度35℃和pH 7.0、底物初始浓度6 g/L、时间36 h、湿细胞浓度10%(w)的条件下，产物的光学纯度高达98.37% e.e.，得率达79.14%. 在上述反应体系中添加5 mmol/L ZnSO4后，重组菌催化的不对称还原反应效率显著提高，产物(R)-苯基乙二醇光学纯度达到100% e.e.，得率高达86.3%.

关键词: 重组大肠杆菌, 生物催化, (R)-苯基乙二醇, 光学纯度, 得率

Abstract: The effects of reaction temperature, pH value, initial substrate concentration, reaction duration, cell concentration and forms on biotransformation reaction for preparation of (R)-1-phenyl-1,2-ethanediol were studied with 2-hydroxyacetophenone and sodium formate as substrates and the whole cells of E. coli Rosetta/pETDuet-rcr-fdh as catalyst .The results showed that when the concentration of 2-hydroxyacetophenone was 6 g/L, (R)-1-phenyl-1,2-ethanediol was produced with high optical purity of 98.37% enantiomeric excess and yield of 79.14% at the conditions of temperature 35℃, pH 7.0, reaction duration 36 h and wet cell concentration 10%(w). Further research showed that the biotransformation efficiency could be well improved by addition of Zn2+. In the presence of Zn2+ with a final concentration of 5 mmol/L, the enantiomeric excess and yield reached 100% and 86.3%, respectively.

Key words: recombinant E. coli strain, biotransformation, (R)-1-phenyl-1, 2-ethanediol, enantiomeric excess, yield

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