Preparation of (R)-1-Phenyl-1,2-ethanediol by Biotransformation with a Coupled System of (R)-Specific Carbonyl Reductase and Formate Dehydrogenase
ZHANG Rong-zhen, XU Yan, DI Ya-wei, WANG Shan-shan, SUN Ying,
Sch.Biotechnology, Jiangnan University Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, SouthenYangtze UniVersity Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, SouthenYangtze UniVersity Sch.Biotechnology, Jiangnan University
Abstract：The effects of reaction temperature, pH value, initial substrate concentration, reaction duration, cell concentration and forms on biotransformation reaction for preparation of (R)-1-phenyl-1,2-ethanediol were studied with 2-hydroxyacetophenone and sodium formate as substrates and the whole cells of E. coli Rosetta/pETDuet-rcr-fdh as catalyst .The results showed that when the concentration of 2-hydroxyacetophenone was 6 g/L, (R)-1-phenyl-1,2-ethanediol was produced with high optical purity of 98.37% enantiomeric excess and yield of 79.14% at the conditions of temperature 35℃, pH 7.0, reaction duration 36 h and wet cell concentration 10%(w). Further research showed that the biotransformation efficiency could be well improved by addition of Zn2+. In the presence of Zn2+ with a final concentration of 5 mmol/L, the enantiomeric excess and yield reached 100% and 86.3%, respectively.
张荣珍 徐岩 耿亚维 王珊珊 孙莹. 羰基还原酶与甲酸脱氢酶偶联催化制备(R)-苯基乙二醇[J]. , 2009, 9(4): 801-805.
ZHANG Rong-zhen; XU Yan; DI Ya-wei; WANG Shan-shan; SUN Ying;. Preparation of (R)-1-Phenyl-1,2-ethanediol by Biotransformation with a Coupled System of (R)-Specific Carbonyl Reductase and Formate Dehydrogenase. , 2009, 9(4): 801-805.
2009, Vol. 9 
