热带假丝酵母及其重组菌产十三碳二元酸的补糖发酵实验

›› 2006, Vol. 6 ›› Issue (5): 814-817.

热带假丝酵母及其重组菌产十三碳二元酸的补糖发酵实验

高弘,刘铭,黄英明,华玉涛,曹竹安

清华大学化学工程系生物化工研究所

出版日期:2006-10-20 发布日期:2006-10-20

Sucrose-added Fed-batch Culture for 1,11-Dicarboxylic Acid Production by Candida tropicalis and Recombinant Candida tropicalis Strain

GAO Hong,LIU Ming,HUANG Ying-ming,HUA Yu-tao,CAO Zhu-an

Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University

Online:2006-10-20 Published:2006-10-20

摘要/Abstract

摘要： 利用热带假丝酵母原始菌F10-1及已构建的肉毒碱乙酰转移酶基因单拷贝缺失重组菌CZ-15，在5 L发酵罐中进行了补糖发酵批式培养，生产十三碳二元酸(DCA13). 结果表明，肉毒碱乙酰转移酶基因单拷贝缺失后，CZ-15菌株的最佳补糖时间和补糖浓度都与原始菌F10-1不同. 采用补糖工艺后，F10-1与CZ-15的十三碳二元酸的产量分别提高了25.0%和23.4%，达到99.5和108.1 g/L，烷烃转化率分别提高了35.5%和28.6%.

关键词: 肉毒碱乙酰转移酶, 十三碳二元酸, 热带假丝酵母, 基因工程菌, 补糖发酵

Abstract: Candida tropicalis strain F10-1 and its recombinant strain CZ-15 can be used for 1,11-dicarboxylic acid (DCA13) production, and in CZ-15, one copy of carnitine acetyltransferase (CAT) genes is disrupted. The sucrose-added fed-batch fermentation of F10-1 and CZ-15 was studied. The results showed that the optimal feeding time and the optimal sucrose concentration were different for F10-1 and CZ-15. By feeding sucrose in the acid production stage, for F10-1 and CZ-15, the acid production was increased by 25.0% and 23.4%, reaching 99.5 and 108.1 g/L, and the alkane conversion rate was increased by 35.5% and 28.6%, respectively.

Key words: carnitine acetyltransferase, DCA13, Candida tropicalis, recombinant strain, fed-batch fermentation

高弘;刘铭;黄英明;华玉涛;曹竹安. 热带假丝酵母及其重组菌产十三碳二元酸的补糖发酵实验[J]. , 2006, 6(5): 814-817.

GAO Hong;LIU Ming;HUANG Ying-ming;HUA Yu-tao;CAO Zhu-an. Sucrose-added Fed-batch Culture for 1,11-Dicarboxylic Acid Production by Candida tropicalis and Recombinant Candida tropicalis Strain[J]. , 2006, 6(5): 814-817.