Preparation of Macroporous Poly(styrene-glycidyl methacrylate) Microspheres with Controlled Pore Structure
LI Yan ZHOU Wei-qing, MA Guang-hui
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
Abstract： Macroporous poly(styrene-glycidyl methacrylate) microspheres [P(ST-GMA)] were prepared using surfactant reverse micelle swelling method. The effects of GMA content, surfactant amount, diluents, and crosslinking agent amount on the pore distribution in microspheres were investigated. The results showed that the pore sizes of microspheres were remarkably increased with increasing of content of GMA. When the surfactant amount was added from 1.6 g to 2.0 g, the pore sizes were correspondingly increased from 100 to 720 nm. Further more, the pore sizes were positively related to the hydrophobicity of diluents. In addition, with the crosslinking agent amount was increased from 1.0 g to 2.0 g, the pore sizes were significantly decreased from 550 to 100 nm. Under the optimization conditions, macroporous P(ST-GMA) microspheres with special pore sizes and different contents of GMA were synthesized. The obtained macroporous microspheres can be used to link various ligands and applied as chromatographic media, or be directly used to immobilize enzymes.