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›› 2002, Vol. 2 ›› Issue (2): 0-0.

• 2 •    

黄孢原毛平革菌选择性合成木质素过氧化物酶和锰过氧化物酶

李华钟，章燕芳，华兆哲，陈坚   

  1. 江南大学生物工程学院工业生物技术教育部重点实验室，江苏 无锡 214036
  • 出版日期:2002-04-20 发布日期:2002-04-20

Investigation on Extraction of Penicillin by Aliphatic Alcohols

WANG Bin, CHEN Ji, LIU Qing-fen, AN Zheng-tao, LIU Hui-zhou   

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, Jiangsu 214036, China
  • Online:2002-04-20 Published:2002-04-20

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被引次数

191

摘要： 在黄孢原毛平革菌(Phanerochaete chrysosporium)的限氮浸没式培养中，研究了不同条件对选择性合成木质素过氧化物酶(LiP)和锰过氧化物酶(MnP)的作用. LiP只在很窄的氮源浓度范围内(0.8~1.8 mmol/L)才能测到，而MnP在较宽浓度范围内(0.4~1.8 mmol/L)表现出较高的酶活水平. 培养基中缺乏Mn2+不能合成MnP，但可以得到活力较高的LiP. [Mn2+]在0.06~0.84 mmol/L时可获得LiP. 添加微量Mn2+即可获得较高活力的MnP，此后增加Mn2+对MnP的活力影响不大，直至浓度为3.36 mmol/L才表现出明显的抑制作用. 通纯氧可使LiP活力提高50%，但对MnP影响不大.

关键词: 黄孢原毛平革菌, 木质素过氧化物酶, 锰过氧化物酶, 选择性合成

Abstract: Selective synthesis of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium in N-limited submerged cultures was studied under different physiological conditions. Whereas lignin peroxidase was detectable only in a narrow range of nitrogen source concentration (0.8~1.8 mmol/L), manganese peroxidase reached considerable levels over a broad range (0.4~2.0 mmol/L). In the absence of Mn2+, only lignin peroxidase was detectable, which was also found when the concentration of Mn2+ was in the range of 0.06~0.84 mmol/L. High level of manganese peroxidase was obtained at very low concentration of Mn2+, and almost kept constant with the increment of Mn2+, but was repressed obviously when the concentration of Mn2+ was beyond 3.36 mmol/L. Pure oxygen increased the activity of lignin peroxidase by 50%, but had no obvious effect on manganese peroxidase.

Key words: aliphatic alcohols, penicillin, extraction

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