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›› 2003, Vol. 3 ›› Issue (2): 0-0.

• 2 •    

羧甲基化单甲氧基聚乙二醇的制备及其对a-干扰素的修饰

胡小剑,何明磊,谭天伟,马光辉   

  1. 1. 北京化工大学化学工程学院,北京 100029;2. 中国科学院过程工程所生化工程国家重点实验室,北京 100080
  • 出版日期:2003-04-20 发布日期:2003-04-20

Catalytic Synthesis and Separation of Ethyl–glucoside

TU Mao-bing, WEI Dong-zhi   

  1. State Key Laboratory of Bioreactor Engineering ECUST , Biochemistry Institute, Shanghai 200237,China
  • Online:2003-04-20 Published:2003-04-20

摘要: 以分子量为5000的单甲氧基聚乙二醇为原料,通过Williamson方法制备了羧甲基化单甲氧基聚乙二醇. 将羧甲基化单甲氧基聚乙二醇活化后,用以修饰重组人a-干扰素. 该修饰剂与蛋白质之间以不易水解的酰胺键连接,是一种较理想的修饰剂. 考察了各种修饰条件对修饰度的影响,修饰结果显示增加修饰剂的用量和提高修饰时的pH值,可以提高修饰度.

关键词: 聚乙二醇, a-干扰素, 化学修饰

Abstract: Research was undertaken to produce ethyl-glucoside by catalysis of ion exchange resin in heterogeneous systems and to determine effects of catalyst type, size, stirring speed and temperature on conversion. 89% of conversion was obtained under the conditions: temperature 78oC, ratio of resin to glucose 1:1, reaction time 20 h. The effective separation of glucoside from glucose was achieved by column chromatography.

Key words: ethyl–glucoside, ion exchange resin, solid catalyst, separation

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