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›› 2002, Vol. 2 ›› Issue (5): 0-0.

• 5 •    

真氧产碱杆菌利用短链有机酸合成聚羟基烷酸酯

严群, 堵国成, 陈坚   

  1. 江南大学工业生物技术教育部重点实验室， 江苏 无锡 214036
  • 出版日期:2002-10-20 发布日期:2002-10-20

Biosynthesis of Polyhydroxyalkanoates by Ralstonia eutropha with Short-chain-length Organic Acids

YAN Qun, DU Guo-cheng, CHEN Jian   

  1. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China
  • Online:2002-10-20 Published:2002-10-20

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被引次数

29

摘要： 研究了真氧产碱杆菌利用单一以及混合短链有机酸作为碳源进行聚羟基烷酸酯(PHAs)的生物合成. 不同的碳氮浓度比对产物的形成有较大影响；在同样的碳源浓度下，较低氮源浓度更加有利于产物的合成；当短链有机酸浓度为8 g/L、硫酸铵浓度为0.3 g/L时对PHAs的合成最为有利.在4种短链有机酸分别单独用作碳源时，乳酸的消耗速度最快，其次为乙酸、丁酸以及丙酸；在单酸发酵条件下，丁酸的PHAs产量最高(2.72 g/L). 通过比较摇瓶以及5 L罐水平的混合酸发酵发现，发酵罐中的各项发酵指标均优于摇瓶. 研究还发现，真氧产碱杆菌在利用丙酸为碳源进行产物合成时，在发酵后期还伴随有乙酸产生.

关键词: 真氧产碱杆菌, 短链有机酸, 聚羟基烷酸酯, PHAs, 生物合成

Abstract: Separation of dimethyl carbonate (DMC) and methanol (MeOH) is very difficult due to the azeotrope of DMC and MeOH, the pressure swing method is an effective method for separation of DMC and MeOH azeotrope. In this paper, a joint process of high-pressure distillation and atmospheric distillation was investigated. Binary interaction parameters of the UNQUAC model between MeOH and DMC were regressed against experimental data of vapor liquid equilibria of MeOH and DMC, the applicable range of the model is: pressure 0.1~1.5 MPa, temperature 337~440 K. Based on the thermodynamic model, the separation flowsheet, inclnding a high-pressure distillation column and an atmospheric distillation column in series, was simulated and analyzed. Sensitivity of operation factors was discussed and the optimum operation parameters were proposed. The optimal parameters of the high-pressure distillation column were as follows: stage number 40, feed stage 29, reflux ratio 7~10, operation pressure 1.0~1.5 MPa.

Key words: Ralstonia eutropha, short-chain-length organic acids, polyhydroxyalkanoates, PHAs, biosynthesis

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