Abstract:The present study was for providing experimental materials for virology, molecular biology and cell biology. Culture of cells from different tissues and the growth of cells in medium with different serum concentration were investigated. A cell line derived from caudal fin of goldfish, Carassius auratus, was developed successfully using explant techniques. The cell line grew well in Medium 199 supplied with 15% FBS at 26oC. The duplication time of the cell line was 3.3 d at a plating density of 2.0′105 cells/ml. It is proved that the cultures in Medium 199 supplied with 15% FBS showed the best result.
贺丽君;戚艺华;聂峰光. 金鱼尾鳍组织的体外培养及血清对其生长的影响[J]. , 2001, 1(3): 0-0.
HE Li-jun; QI Yi-hua; NIE Feng-guang. Culture of Cells from Caudal Fin of Goldfish and the Effect of Serum on Cell Growth. , 2001, 1(3): 0-0.