A Rapid Screening Method of Producing Strain in Acetone-Butanol Fermentation
JIN Xiao-qing ZHOU Hua WU Xue-ming ZHANG Gan-dao HE Bing-fang
College of Life Science and pharmaceutical engineering, Nanjing University of Technology College of Life Science and pharmaceutical engineering, Nanjing University of Technology College of Life Science and Pharmacy, Nanjing University of Technology, The Provincial Key Laboratory of Industrial Biotechnology
Abstract：A rapid screening method of producing strains in acetone-butanol fermentation was developed. Nitrogen ion beam implantation was used to breed producing strains in the fermentation. The high amylase activity mutants were obtained by using 2-deoxy-D-glucose as the antimetabolite. Then the high-yielding mutants were further screened by screening plates of bromocresol green and resazurin. It was proved that the butanol and solvent producing ability of selected mutants were significantly improved. The total solvents production and butanol production of mutant I4-28 increased by 27.96% and 40.66%, compared with the parent strain, and the ratio of butanol to total solvents was up to 71.94% from 63.39%. The mutant had good genetic stability after 8 times of subculture. The rapid screening method was simple, efficient and significantly valuable in breeding of acetone-butanol producing strains.