Abstract：The recombinant bacterium F6 was constructed with the recombinant plasmid pDK7-fdh-parDE which contained the genes parDE and fdh. While the FY and F-1 were constructed with the recombinant plasmids pDK6-fdh and pMAL-p2X-fdh only containing fdh, respectively. K. oxytoca M5al is the initial bacterium of F6, FY and F-1. The effect of parDE on plasmid stability and FDH (formate dehydrogenase) enzyme activity was also studied. It was shown that 93% F-1 and 78% FY lost the plasmids while only 3% F6 lost the plasmids after 160 generations. Furthermore, the FDH specific enzyme activity of F6 decreased from 2.82 to 2.77 U/mg, only 1.77% decreased, while the enzyme activity of FY decreased significantly from 2.69 to 2.02 U/mg, and F-1 decreased from 2.63 to 1.33 U/mg, close to the enzyme activity of M5al after 160 generations. It was indicated that the recombinant bacteria with the parDE had obvious advantage on plasmid stability, and FDH enzyme activity was higher than the recombinant bacteria without the parDE.
张刚 杨光 裴海生 李春 曹竹安. 质粒平均分配基因parDE对甲酸脱氢酶NADH再生系统稳定性的影响[J]. , 2008, 8(2): 345-349.
ZHANG Gang YANG Guang LI Chun CAO Zhu-an. Effect of Plasmid Distribution Gene parDE on the Stability of Formate Dehydrogenase NADH Regeneration System. , 2008, 8(2): 345-349.