1. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2. Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
3. Qingdao Institute of Bioenergy and Bioprocess Technology, China Academy of Science
Abstract：A novel method to fast characterize cell differentiation of Haematococcus pluvialis SCCAP k-0084 by cell osmotic sensitivity of motile cells and non-motile cells on Triton X-100 was proposed and then cell propagation and differentiation of H. pluvialis in two fresh medium BG11 and BBM were investigated. It was found that BBM medium kept a high percentage of motile cells in the first 8 d cultivation. In contrast, the released motile cells gradually transformed to non-motile cells. The cell proliferation in BBM medium is contributed by both mitosis and the formation of sporangium of motile cells. It is proved that such differences of cell propagation and differentiation are produced by that the low phosphorus concentration in BG11, indicating the importance of appropriate phosphorus concentration in medium to maintain the continuous division of motile cells.
徐晓莹 程天佑 陈林 张维 刘天中. 两种培养基间雨生红球藻细胞生长分化差异及磷的作用[J]. 过程工程学报, 2016, 16(5): 840-848.
XU Xiao-ying CHENG Tian-you CHEN Lin ZHANG Wei LIU Tian-zhong,. Effects of Phosphorus on H. pluvialis Cell Propagation and Differentiation in Two Medium. Chin. J. Process Eng., 2016, 16(5): 840-848.