ZHU Kai 1,ZHAO Lan 2,HUANG Yong-dong, 3,LI Qiang 4,QIU Han 5,WANG Qi-bao 6,SU Zhi-guo 7,MA Guang-hui 8
1. China University of Mining and Technology, Beijing., School of Chemical and Environment Engineering
2. State Key Lab.Biochem.Eng.,Institute of Process Engineering, Chinese Academy of Sciences
3. State Key laboratory of Biochemical Engineering, Institute of Process Engineering, CAS
4. National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences
5. Department of Applied Chemistry, Beijing Institute of Petrochemical Technolog
6. College of Chemical and Environmental Engineering,China University of Mining and Technology
7. Key Laboratory of Biochemical Engineering，Institute of Process Engineering，Chinese Academy of Sciences
8. State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
Abstract：After being activated by epoxy groups, Sepharose 4FF was grafted with dextran and then epoxy-activated followed by being coupled with alkali-resistant protein A ligand. In this paper, the cleaning-in-place performance of the medium is measured, and thermodynamic studies were carried out. Compared with non-grafted medium, the maximum velocity of dextran-grafted protein A medium was increased by about 32%. The binding capacity of antibody hIgG of dextran-grafted protein A medium was 60.6 mg/mL, which has reached 123% and 95% of the values for non-grafted protein A and MabSelect SuRe. After 40 cleaning-in-place cycles rinsed, the dynamic binding capacity of dextran-grafted protein A maintained the original capacity of 92%, which was almost the same as MabSelect SuRe and was also much higher than non-grafted Protein A. The capacity of non-grafted protein A maintained the original capacity of 84%. Thermodynamic studies showed that the binding processes of three chromatographic media were entropy-driven processes. The adsorption heat of dextran-grafted protein A medium was between that of MabSelect SuRe and non-grafted protein A.
朱凯 赵岚 黄永东 李强 邱晗 王启宝 苏志国 马光辉. 葡聚糖接枝型耐碱Protein A介质的层析性能提升研究[J]. 过程工程学报, 2016, 16(5): 856-861.
ZHU Kai ZHAO Lan HUANG Yong-dong, LI Qiang QIU Han WANG Qi-bao SU Zhi-guo MA Guang-hui. . Chin. J. Process Eng., 2016, 16(5): 856-861.