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›› 2005, Vol. 5 ›› Issue (3): 337-340.

• 生化工程专栏 • 上一篇    下一篇

添加甲萘醌促进嗜热子囊菌合成过氧化氢酶

王明星,李寅,方芳,华兆哲,陈坚   

  1. 江南大学生物工程学院环境生物技术研究室
  • 出版日期:2005-06-20 发布日期:2005-06-20

Addition of Menadione for Stimulating Catalase Accumulation by Thermoascus aurantiacus

WANG Ming-xing,LI Yin,FANG Fang,HUA Zhao-zhe,CHEN Jian   

  1. Lab. Envionmntal Biotech., School of Biotech., Southern Yangtze University
  • Online:2005-06-20 Published:2005-06-20

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摘要： 研究了添加甲萘醌对嗜热子囊菌(Thermoascus aurantiacus)IFO9862合成过氧化氢酶(CAT)的影响. 结果表明，添加甲萘醌有助于促进CAT的合成. 在发酵的24或48 h分别添加1 mmol/L的甲萘醌，发酵终了CAT酶活比对照分别提高了11.0%和23.1%；而在发酵的36和48 h各添加1 mmol/L的甲萘醌，CAT酶活可提高32.5%. 在发酵0 h添加88 mmol/L的H2O2，同时在48 h添加1 mmol/L的甲萘醌，CAT酶活达到1725 U/mL，比对照(1074 U/mL)提高了60.6%.

关键词: 过氧化氢酶, 甲萘醌, 过氧化氢, 嗜热子囊菌

Abstract: The effect of menadione on the accumulation of CAT by Thermoascus aurantiacus was investigated. The experimental results indicate that addition of menadione helps to the accumulation of CAT. When 1 mmol/L menadione was added to fermentation liquor at 24 or 48 h, CAT activity in culture broth increased 11.0% and 23.1%, compared with that of control. CAT activity increased 32.5% when menadione was added at 36 and 48 h. CAT activity reached 1725 U/mL when 88 mmol/L hydrogen peroxide was added at 0 h and 1 mmol/L menadione was added at 48 h, which was 60.6% higher than that of control (1074 U/mL).

Key words: catalase, menadione, hydrogen peroxide, Thermoascus aurantiacus