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过程工程学报 ›› 2018, Vol. 18 ›› Issue (2): 427-433.DOI: 10.12034/j.issn.1009-606X.217123

• 生化工程 • 上一篇    下一篇

基于酶解-膜分离集成的胶原蛋白肽分子量控制技术

崔 浩1， 牟玉洁1，王 凯1， 康跻耀2，张贵锋2， 王明林1*     

  1. 1. 山东农业大学食品科学与程学院，山东 泰安 250100；2. 中国科学院过程工程研究所生化工程国家重点实验室，北京 100190
  • 收稿日期:2017-02-06 修回日期:2017-08-15 出版日期:2018-04-22 发布日期:2018-04-10
  • 通讯作者: 王明林 mlwang@sdau.edu.cn
  • 基金资助:
    广东省自然科学基金

Preparation of Collagen Peptides with Controllable Molecular Weight Range Based on Enzymatic Degradation Coupled with Membrane Separation

Hao CUI1,  Yujie MOU1,  Kai WANG1,  Jiyao KANG2,  Guifeng ZHANG2,  Minglin WANG1*   

  1. 1. College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 250100, China; 2. State Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2017-02-06 Revised:2017-08-15 Online:2018-04-22 Published:2018-04-10
  • Contact: WANG Ming-lin mlwang@sdau.edu.cn
  • Supported by:
    Natural Science Foundation of Guangdong Province

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摘要： 采用酶解?膜分离集成工艺制备分子量范围可控的胶原蛋白多肽，研究反应过程中酶种类、截留分子量和过滤体积等因素对酶解?膜分离集成工艺的影响. 结果表明，用截留分子量为3 kDa的超滤膜处理的透过液分子量主要分布在4.0, 1.6和0.6 kDa，比例分别为13.7%, 34.8%和51.4%；用截留分子量为8 kDa的超滤膜处理的透过液分子量主要分布在8.3, 4.0, 1.6和0.6 kDa，比例分别为14.5%, 22.7%, 37.7%和25.1%；酶解?膜分离集成工艺蛋白转化速率比酶解过程提高15%，表明酶解?膜分离集成可以加快大分子蛋白转化率，并使酶解产物分子量均一可控.

关键词: 胶原蛋白肽, 酶解, 膜分离, 过程集成, 高效凝胶色谱

Abstract: Collagen peptides with controllable molecular weight range were prepared by using enzymatic hydrolysis coupled with membrane separation. The effects of enzyme type, molecular weight and volume of filtration on the enzymolysis?membrane separation process was investigated. The results showed that the molecular weight of the permeated liquid treated with 3 kDa ultrafiltration membrane was mainly distributed in 4.0, 1.6 and 0.6 kDa, share ratio were 13.7%, 34.8% and 51.4%. The molecular weight of the permeated liquid treated with 8 kDa ultrafiltration membrane was mainly distributed in 8.3, 4.0, 1.6 and 0.6 kDa, share ratio were 14.5%, 22.7%, 37.7% and 25.1%. It was found that the protein conversion rate was increased by 15% compared with the traditional enzymatic hydrolysis process, thus, enzymatic degradation coupled membrane separation is possible strategy for preparation of uniform molecular weight collagen peptide.

Key words: Collagen peptides, Enzymatic degradation, Membrane separation, Process Integration, size exclusion chromatography